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Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor 期刊论文

编号：

009e3de8-d780-49f3-ac9a-c47554135978
作者：

Li, ChangZheng^[1,2,3];Liang, ZhongKun^[1];Chen, ZhenRui^[1];Lou, HaiBo^[1];Zhou, Ye^[2];Zhang, ZheHuan^[1];Yu, Fei^[4];Liu, Shuwen^[4];Zhou, Yuanping^[1];Wu, Shuguang^[4];Zheng, Wenling^[2];Tan, Wanlong^[1];Jiang, Shibo(姜世勃)^[5]Zhou, Chen^[4,6];
语种：

English
期刊：

CELLULAR & MOLECULAR IMMUNOLOGY ISSN：1672-7681 2012 年 9 卷 2 期 (184 - 190) ; MAR
收录：
关键词：

antibody display antibody screening full-length antibody HBsAg-specific antibody mammalian display
摘要：

Hepatitis B immunoglobulin (HBIG) is important in the management of hepatitis B virus (HBV) infection. Aiming to develop recombinant monoclonal antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen (HBsAg)-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the Flp recombinase-mediated integration (Flp-In) system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting (FACS), eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression (FSE), fluorescent strength of binding (FSB) and relative binding ability (RBA) were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies. Cellular & Molecular Immunology (2012) 9, 184-190; doi: 10.1038/cmi.2011.55; published online 19 December 2011
推荐引用方式
GB/T 7714：

Li Chang-Zheng,Liang Zhong-Kun,Chen Zhen-Rui, et al. Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor [J].CELLULAR & MOLECULAR IMMUNOLOGY,2012,9(2):184-190.
APA：

Li Chang-Zheng,Liang Zhong-Kun,Chen Zhen-Rui,Lou Hai-Bo,&Zhou Chen.(2012).Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor .CELLULAR & MOLECULAR IMMUNOLOGY,9(2):184-190.
MLA：

Li Chang-Zheng, et al. "Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor" .CELLULAR & MOLECULAR IMMUNOLOGY 9,2(2012):184-190.

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