[1]Southeast University, State Key Laboratory of Bioelectronics,Nanjing,China
[2]Shaoguan University, College of Chemistry and Environmental Engineering,Shaoguan,China
[3]Hospital of Nanjing Medical University, Department of Cardiology,Nanjing,China
[4]Hospital of Nanjing Medical University, Department of Laboratory Medicine,Nanjing,China
[5]Fudan University, Department of Endocrinology,Shanghai,China
A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.