The dissemination, seeding, and colonization of circulating tumor cells (CTCs) serve as the root of distant metastasis. As a key step in the early stage of metastasis formation, colonization of CTCs in the (pre)-metastatic niche appears to be a valuable target. Evidence showed that inflammatory neutrophils possess both a CTCand niche-targeting property by the intrinsic cell adhesion molecules on neutrophils. Inspired by this mechanism, we developed a nanosize neutrophil-mimicking drug delivery system (NM-NP) by coating neutrophils membranes on the surface of poly(latic-co-glycolic acid) nanoparticles (NPs). The membrane-associated protein cocktails on neutrophils membrane were mostly translocated to the surface of NM-NP via a nondisruptive approach, and the biobinding activity of neutrophils was highly preserved. Compared with uncoated NP, NM-NP exhibited enhanced cellular association in 4T1 cell models under shear flow in vitro, much higher CTC-capture efficiency in vivo, and improved homing to the premetastatic niche. Following loading with carfilzomib, a second generation of proteasome inhibitor, the NM-NP-based nanoformulation (NM-NP-CFZ) selectively depleted CTCs in the blood, prevented early metastasis and potentially inhibited the progress of already-formed metastasis. Our NP design can neutralize CTCs in the circulation and inhibit the formation of a metastatic niche.
[1]Fudan Univ, Sch Pharm, Minist Educ, Key Lab Smart Drug Delivery, Lane 826,Zhangheng Rd, Shanghai 201203, Peoples R China.
[2]Shanghai Jiao Tong Univ, Med Res Inst X, 1954 Huashan Rd, Shanghai 200030, Peoples R China.
[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, 1954 Huashan Rd, Shanghai 200030, Peoples R China.
[4]Shanghai Jiao Tong Univ, Sch Med, Inst Med Sci, Dept Pharmacol, 280 South Chongqing Rd, Shanghai 200025, Peoples R China.
(8.0+极速模式)