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Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery 期刊论文

编号：

06d279ed-c13f-4fd7-891e-dd282c36b2fa
作者：

Zhang, Zhiwen(张志文)#^[1]Sha, Xianyi(沙先谊)*^[1]Shen, Anle^[1];Wang, Yongzhong(王永中)^[1]Sun, Zhaogui^[2];Gu, Zheng^[2];Fang, Xiaoling(方晓玲)^[1]
语种：

English
期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSN：0006-291X 2008 年 370 卷 3 期 (478 - 482) ; JUN 6
收录：
关键词：

polycation nanostructured lipid carrier triolein nonviral vectors polyethylenimine gene delivery
摘要：

A novel nonviral gene transfer vector was developed by modifying nanostructured lipid carrier (NLC) with cetylated polyethylenimine (PEI). Polycation nanostructured lipid carrier (PNLC) was prepared using the emulsion-solvent evaporation method. Its in vitro gene transfer properties were evaluated in the human lung adenocarcinoma cell line SPC-A1 and Chinese Hamster Ovary (CHO) cells. Enhanced transfection efficiency of PNLC was observed after the addition of triolein to the PNLC formulation and the transfection efficiency of the optimized PNLC was comparable to that of Lipofectamine (TM) 2000. In the presence of 10% serum the transfection efficiency of the optimal PNLC was not significantly changed in either cell line, whereas that of Lipofectamine (TM) 2000 was greatly decreased in both. Thus, PNLC is an effective nonviral gene transfer vector and the gene delivery activity of PNLC was enhanced after triolein was included into the PNLC formulation. (c) 2008 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Zhang Zhiwen,Sha Xianyi,Shen Anle, et al. Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery [J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,370(3):478-482.
APA：

Zhang Zhiwen,Sha Xianyi,Shen Anle,Wang Yongzhong,&Fang Xiaoling.(2008).Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,370(3):478-482.
MLA：

Zhang Zhiwen, et al. "Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery" .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 370,3(2008):478-482.

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