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Gene expression profiling reveals the defining features of monocytes from septic patients with compensatory anti-inflammatory response syndrome 期刊论文

编号：

06f9ef82-9420-4a91-b323-40379499753d
作者：

Xu, Pingbo(许平波)#^[1]Lou, JingSheng^[2];Ren, Yu(任瑜)^[1]Miao, Changhong(缪长虹)*^[1]Deng, Xiaoming^[2];
语种：

English
期刊：

JOURNAL OF INFECTION ISSN：0163-4453 2012 年 65 卷 5 期 (380 - 391) ; NOV
收录：
关键词：

Sepsis Septic shock Systemic inflammatory response syndrome Compensatory anti-inflammatory response syndrome Monocyte deactivation
摘要：

Objectives: To characterize the expression profiles of genes in purified monocytes from septic patients during systemic inflammatory response syndrome (SIRS) and compensatory anti-inflammatory response syndrome (CARS), and then to investigate the potential mechanism of monocyte deactivation. Methods: Lipopolysaccharides (LPS)-induced cytokine responses, phagocytosis assay and migration assay were performed in monocytes from SIRS patients, CARS patients and healthy volunteers (n = 8). After functional assays, each pair of samples from the same group was pooled into one for gene expression analysis. All new samples (n = 4) were hybridized on NimbleGen human gene expression 12 x 135 K microarrays, and selected genes were validated by real-time polymerase chain reaction. Pathway analysis and Gene Ontology analysis were performed on differentially expressed genes using Agilent GeneSpring (version 11.0). Results: A set of genes related to pro-inflammation, phagocytosis, chemotaxis, antigen presentation, and anti-apoptosis were significantly down-regulated, while some genes associated with pro-apoptosis and anti-inflammation were up-regulated instead on monocytes from CARS patients compared with SIRS patients and healthy volunteers. Monocytes from CARS patients showed impaired production of TNF-alpha and IL-6, and increased release of IL-10 when stimulated by LPS. Functional analysis confirmed reduced phagocytosis and migratory activity of monocytes from CARS patients. Human leukocyte antigen-DR (HLA-DR) measurements demonstrated decreased expression of HLA-DR on monocytes from CARS patients. Conclusion: Monocytes from CARS patients exhibited significant changes in mRNA expression of genes associated with phagocytosis, antigen presentation, inflammatory response, cell migration, and apoptosis, which might cause deactivation of monocytes during CARS. (c) 2012 The British Infection Association. Published by Elsevier Ltd. All rights reserved.
推荐引用方式
GB/T 7714：

Xu Ping-bo,Lou Jing-Sheng,Ren Yu, et al. Gene expression profiling reveals the defining features of monocytes from septic patients with compensatory anti-inflammatory response syndrome [J].JOURNAL OF INFECTION,2012,65(5):380-391.
APA：

Xu Ping-bo,Lou Jing-Sheng,Ren Yu,Miao Chang-hong,&Deng Xiao-ming.(2012).Gene expression profiling reveals the defining features of monocytes from septic patients with compensatory anti-inflammatory response syndrome .JOURNAL OF INFECTION,65(5):380-391.
MLA：

Xu Ping-bo, et al. "Gene expression profiling reveals the defining features of monocytes from septic patients with compensatory anti-inflammatory response syndrome" .JOURNAL OF INFECTION 65,5(2012):380-391.

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