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MiR-10a and MiR-10b Target the 3 '-Untranslated Region of TBX5 to Repress Its Expression 期刊论文

编号：

0815d195-52da-4ced-9032-3b6243387c00
作者：

Wang, Feng^[1];Yang, XueYan^[2,3];Zhao, JianYuan^[2,3];Yu, LiWei^[1];Zhang, Ping^[1];Duan, WenYuan^[4];Chong, Mei^[5];Gui, YongHao(桂永浩)*^[1]
语种：

English
期刊：

PEDIATRIC CARDIOLOGY ISSN：0172-0643 2014 年 35 卷 6 期 (1072 - 1079) ; AUG
收录：
关键词：

Congenital heart disease TBX5 3 '-UTR MiRNAs
摘要：

As a well-known transcription factor, TBX5 is involved in embryonic cardiac development. Although TBX5 functions in a dose-dependent manner, the posttranscriptional regulation of human TBX5 is poorly understood. Thus, this study aimed to identify microRNAs that modulate TBX5 expression. Luciferase assays were used to screen miRNAs predicted to modulate TBX5 expression. Using quantitative reverse transcriptase-polymerase chain reaction and Western blot analysis, the authors found that miR-10a and miR-10b significantly repressed TBX5 expression and decreased TBX5 protein levels by targeting the TBX5 3'-untranslated region. In addition, miR-10a and miR-10b expression levels were respectively 2.77 and 3.51 times higher in the heart tissues of congenital heart disease patients than in healthy control subjects, suggesting that they are potential diagnostic biomarkers. In conclusion, the study results indicate that miR-10a and miR-10b inhibit TBX5 expression at the level of translation. Higher levels of miR-10a and miR-10b expression are associated with a higher risk of congenital heart defects.
推荐引用方式
GB/T 7714：

Wang Feng,Yang Xue-Yan,Zhao Jian-Yuan, et al. MiR-10a and MiR-10b Target the 3 '-Untranslated Region of TBX5 to Repress Its Expression [J].PEDIATRIC CARDIOLOGY,2014,35(6):1072-1079.
APA：

Wang Feng,Yang Xue-Yan,Zhao Jian-Yuan,Yu Li-Wei,&Gui Yong-Hao.(2014).MiR-10a and MiR-10b Target the 3 '-Untranslated Region of TBX5 to Repress Its Expression .PEDIATRIC CARDIOLOGY,35(6):1072-1079.
MLA：

Wang Feng, et al. "MiR-10a and MiR-10b Target the 3 '-Untranslated Region of TBX5 to Repress Its Expression" .PEDIATRIC CARDIOLOGY 35,6(2014):1072-1079.

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