Background: The ARABIDOPSIS SKP1-LIKE1 (ASK1) protein functions as a subunit of SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases. Previous genetic studies showed that ASK1 plays important roles in Arabidopsis flower development and male meiosis. However, the molecular impact of ASK1-containing SCF E3 ubiquitin ligases (ASK1-E3s) on the floral proteome and transcriptome is unknown. Results: Here we identified proteins that are potentially regulated by ASK1-E3s by comparing floral bud proteomes of wild-type and the ask1 mutant plants. More than 200 proteins were detected in the ask1 mutant but not in wild-type and >300 were detected at higher levels in the ask1 mutant than in wild-type, but their RNA levels were not significantly different between wild-type and ask1 floral buds as shown by transcriptomics analysis, suggesting that they are likely regulated at the protein level by ASK1-E3s. Integrated analyses of floral proteomics and transcriptomics of ask1 and wild-type uncovered several potential aspects of ASK1-E3 functions, including regulation of transcription regulators, kinases, peptidases, and ribosomal proteins, with implications on possible mechanisms of ASK1-E3 functions in floral development. Conclusions: Our results suggested that ASK1-E3s play important roles in Arabidopsis protein degradation during flower development. This study opens up new possibilities for further functional studies of these candidate E3 substrates.
[1]Penn State Univ, Huck Inst Life Sci, Intercoll Grad Degree Program Plant Biol, University Pk, PA 16802 USA.
[2]Penn State Univ, Dept Biol, University Pk, PA 16802 USA.
[3]Penn State Univ, Sect Res Resources, Coll Med, Hershey, PA 17033 USA.
[4]Fudan Univ, Sch Life Sci, Ctr Evolutionary Biol, State Key Lab Genet Engn, Shanghai 200433, Peoples R China.
[5]Fudan Univ, Sch Life Sci, Ctr Evolutionary Biol, Inst Plant Biol, 4, Shanghai 200433, Peoples R China.
[6]Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA.
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