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Nonselective Cyclooxygenase Inhibition Retards Cyst Progression in a Murine Model of Autosomal Dominant Polycystic Kidney Disease 期刊论文

编号：

0c4d0947-2598-4e1f-856e-063a6f283240
作者：

Zhang, Min(张敏)#^[1]Srichai, Manakan B.^[2,4];Zhao, Min^[2];Chen, Jian^[2];Davis, Linda S.^[2];Wu, Guanqing^[2,3];Breyer, Matthew D.^[5];Hao, ChuanMing(郝传明)*^[1,2,4]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ISSN：1449-1907 2019 年 16 卷 1 期 (180 - 188)
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关键词：

ADPKD COX2 PKD2 prostanoid Sulindac
摘要：

Aim: Autosomal dominant polycystic kidney disease is one of the most common genetic renal diseases. Cyclooxygenase plays an important role in epithelial cell proliferation and may contribute to the mechanisms underlying cyst formation. The aim of the present study was to evaluate the role of cyclooxygenase inhibition in the cyst progression in polycystic kidney disease.
Method: Pkd2(ws25/-) mice, a murine model which harbors a compound cis-heterozygous mutation of the Pkd2 gene were used. Cyclooxygenase expression was assessed in both human and murine kidney specimens. Pkd2(ws25/-) mice were treated with Sulindac (a nonselective cyclooxygenase inhibitor) or vehicle for 8 months starting at three weeks age, and then renal cyst burden was assessed by kidney weight and volume.
Results: Cyclooxygenase-2 expression was up-regulated compared to control kidneys as shown by RNase protection in human polycystic kidneys and immunoblot in mouse Pkd2(ws25/-) kidneys. Cyclooxygenase-2 expression was up-regulated in the renal interstitium as well as focal areas of the cystic epithelium (p<0.05). Basal Cyclooxygenase-1 levels were unchanged in both immunohistochemistry and real-time PCR. Administration of Sulindac to Pkd2(ws25/-) mice and to control mice for 8 months resulted in reduced kidney weights and volume in cystic mice. Renal function and electrolytes were not significantly different between groups.
Conclusion: Thus treatment of a murine model of polycystic kidney disease with Sulindac results in decreased kidney cyst burden. These findings provide additional implications for the use of Cyclooxygenase inhibition as treatment to slow the progression of cyst burden in patients with polycystic kidney disease.
推荐引用方式
GB/T 7714：

Zhang Min,Srichai Manakan B.,Zhao Min, et al. Nonselective Cyclooxygenase Inhibition Retards Cyst Progression in a Murine Model of Autosomal Dominant Polycystic Kidney Disease [J].INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,2019,16(1):180-188.
APA：

Zhang Min,Srichai Manakan B.,Zhao Min,Chen Jian,&Hao Chuan-Ming.(2019).Nonselective Cyclooxygenase Inhibition Retards Cyst Progression in a Murine Model of Autosomal Dominant Polycystic Kidney Disease .INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,16(1):180-188.
MLA：

Zhang Min, et al. "Nonselective Cyclooxygenase Inhibition Retards Cyst Progression in a Murine Model of Autosomal Dominant Polycystic Kidney Disease" .INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 16,1(2019):180-188.
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