[1]Dako North America, Inc.,Carpinteria,United States
[2]Roswell Park Cancer Institute, Department of Flow and Image Cytometry,Buffalo,United States
[3]Roswell Park Cancer Institute, Department of Pharmacology and Therapeutics,Buffalo,United States
[4]Agilent Technologies, Dako North America,Santa Clara,United States
[5]Dako AS,Glostrup,Denmark
[6]Northwest Normal University, College of Geography and Environmental Science,Lanzhou,China
[7]University of Nebraska Medical Center, Department of Biochemistry and Molecular Biology and Eppley Institute for Research in Cancer and Allied Diseases and Fred and Pamela Buffett Cancer Center,Omaha,United States
[8]Fudan University School of Life Sciences, Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Shanghai,China
Aims: To elucidate the expression and regulation of survivin in normal tissues. Methods and results: A novel monoclonal antibody (12C4) to survivin was generated. Application of this antibody to determine survivin expression in human normal adult tissues revealed that most adult tissues do not express survivin and, where it is present, survivin is largely restricted to a small subset of epithelial cells and cells with proliferative potential such as thymus. Survivin expression among positive tissues showed individual variations, ranging from zero to < 5% positive cells in epithelial cell populations. Testis is the only human adult tissue highly expressing survivin, with 60-70% positivity in the nuclei of spermatogonia. Consistent with deregulated expression of survivin associated with oncogenesis, we found that certain ligands and transcription factors differentially modulate survivin promoter activity in cancer cells versus normal/untransformed cells. Conclusion: Deregulation of survivin transcription controls in individual epithelial cells may contribute to oncogenesis in various human adult tissues. © 2006 The Authors.
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