It was previously demonstrated that anomalous expression of indoleamine 2,3-dioxygenase-1 (IDO1) in endometrial stromal cells (ESCs) stimulated an inflammatory response that subsequently initiated the activation of immunosuppressive macrophages in endometriosis. The aim of the present study was to clarify the effect of IDO1-induced macrophages on the growth of ESCs in endometriosis. Normal ESCs, ectopic ESCs and normal ESCs treated with plasmid pEGFP-N1-IDO1 or SD11-IDO1 short hairpin RNA were co-cultured with peripheral blood-derived monocyte (PBMC)-driven macrophages directly for 48 h. Compared with normal ESCs, the PBMC-driven macrophages that were co-cultured with ectopic ESCs displayed a lower phagocytic ability. pEGFP-N1-IDO1 transfection of normal ESCs also decreased the phagocytic ability of co-cultured macrophages. Additionally, pEGFP-N1-IDO1-transfected ESC-induced macrophages significantly increased the viability and proliferation of ESCs, while ESC apoptosis was decreased, compared with control ESCs. In conclusion, IDO1 educated-macrophages may facilitate the survival of retrograde endometrial tissues, and be involved in the pathogenesis of endometriosis.
[1]Nanjing Univ, Sch Med, Reprod Med Ctr,Dept Obstet & Gynecol, Nanjing Drum Tower Hosp,Affiliated Hosp, 321 Zhong Shan Rd, Nanjing 210008, Jiangsu, Peoples R China.
[2]Fudan Univ, Shanghai Med Sch, Hosp & Inst Obstet & Gynecol, Shanghai 200011, Peoples R China.
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