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In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-kappa B signaling pathway 期刊论文

编号：

0fd9a693-935f-49ef-adcd-293ac634934f
作者：

Cai, QiHang^[1] Tang, Yao^[1] Fan, ShaoHua^[1] Zhang, ZiFeng^[1] Li, Hong^[2] Huang, ShaoQiang^[2] Wu, DongMei^[1] Lu, Jun^[1] Zheng, YuanLin^[1]
语种：

English
期刊：

BIOMEDICINE & PHARMACOTHERAPY ISSN：0753-3322 2017 年 95 卷 (1830 - 1837) ; NOV
收录：
关键词：

Ovarian cancer Dexmedetomidine P38MAPK/NF-kappa B signaling pathway Immune function Tumor growth
摘要：

Objectives: During this study, we aimed to analyze the correlation between dosages of dexmedetomidine (DEX) and the p38MAPK/NF-kappa B signaling pathway, and their effects on immune function and tumor growth in rats with ovarian cancer (OC). Methods: A total of 100 rats were selected for the purposes of the study. The normal group consisted of 20 rats, while the remaining 80 rats were utilized for OC model establishment purposes, and further assigned into the model, 0.2 DEX, 1 DEX and 5 DEX groups (based on respective dosages of DEX, n= 20 per group). The tumor inhibition rate was calculated. Positive expressions of p38 and NF-kappa B in ovarian tissues were examined by means of immunohistochemical staining. Cell transformation as well as lymphocyte proliferation rates were measured using MTT. Cell cycle and apoptosis of CD4(+) and CD8(+) cells were determined by flow cytometry. Serum levels of IL-2 and TNF-alpha were detected using ELISA, while qRT-PCR and western blotting methods were used to analyze mRNA and protein expressions of p38 and NF-kappa B. Results: Compared with the normal group, the other four groups exhibited up-regulated IL-2, TNF-alpha serum levels as well as up regulated expressions of p38, NF-kappa B65 mRNA and protein; while the respective percentages of both CD4(+) and CD8(+) T cells exhibited down-regulated rates. The other four groups displayed increases in tumor weight and cell apoptosis, as well as decreased levels of cell proliferation and transformation rates. The aforementioned findings of the study ultimately highlighted a greater tendency among the three DEX groups in comparison to the model group. Conclusion: The findings of the study suggest that a particular dosage of DEX may act to enhance the immune function of rats with OC by inhibiting the p38MAPK/NF-kappa B signaling pathway.
推荐引用方式
GB/T 7714：

Cai Qi-Hang,Tang Yao,Fan Shao-Hua, et al. In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-kappa B signaling pathway [J].BIOMEDICINE & PHARMACOTHERAPY,2017,95:1830-1837.
APA：

Cai Qi-Hang,Tang Yao,Fan Shao-Hua,Zhang Zi-Feng,&Zheng Yuan-Lin.(2017).In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-kappa B signaling pathway .BIOMEDICINE & PHARMACOTHERAPY,95:1830-1837.
MLA：

Cai Qi-Hang, et al. "In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-kappa B signaling pathway" .BIOMEDICINE & PHARMACOTHERAPY 95(2017):1830-1837.

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