Background Extramammary Paget's disease (EMPD) is a rare skin cancer with relative high frequencies of germline and somatic mismatch repair (MMR) genes mutations. However, the methylation and expression of these genes have not been validated in EMPD.
Objective This study aims to confirm the methylation and expression of MMR genes in EMPD.
Methods Immunohistochemical (IHC) staining detection and Methylation-specific PCR (MSP) were used to analyse MLH1, MSH2, MSH6 and PMS2 proteins' expression and promoters' methylation in 57 EMMD samples, and pyrosequence was used to find highly methylated CpG sites in MSH2 promoter.
Results Immunohistochemical detection displayed reduced expression of MSH2 in 38.6% EMPD cases but normal expression of MLH1, MSH6 and PMS2 in all tumour tissues. Hypermethylation also was found in the promoter of MSH2 but not in other MMR genes. Pyrosequencing of MSH2 promoter showed CpG6 (-87) and CpG3 (-98) were the most common two methylated CpG dinucleotides. There is a significant correlation between reduced MSH2 expression and MSH2 methylation.
Conclusion Reduced MSH2 expression and hypermethylation in this gene promoter were common genetic changes in EMPD, which expands our understanding of the role of MMR function in this skin cancer.
[1]Fudan Univ, Huashan Hosp, Dept Lab Med, Shanghai, Peoples R China;
[2]Fudan Univ, Huashan Hosp North, Dept Pathol, Shanghai, Peoples R China;
[3]Fudan Univ, Huashan Hosp, Dept Dermatol, Shanghai, Peoples R China;
[4]Fudan Univ, Huashan Hosp, Cent Lab, Shanghai, Peoples R China
(8.0+极速模式)