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Surveillance of shigellosis by real-time PCR suggests underestimation of shigellosis prevalence by culture-based methods in a population of rural China 期刊论文

编号：

0fef8ccd-d7e0-4a9a-ad7a-86a68e65242b
作者：

Wang, SongMei^[2];Ma, JingChen^[6];Hao, ZhiYong^[6];Zhang, ZhiYong^[6];Mason, Carl^[5];Sethabutr, Ornthipa^[5];von Seidlein, Lorenz^[4];Wang, XuanYi(汪萱怡)*^[1,3]Xu, ZhiYi^[4];
语种：

English
期刊：

JOURNAL OF INFECTION ISSN：0163-4453 2010 年 61 卷 6 期 (471 - 475) ; DEC
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关键词：

Disease burden Shigella Real-time PCR ipaH Culture
摘要：

Introduction: Shigellosis is a leading public health issue in China, especially in Children under 5 years of age. The disease burden of shigellosis is usually underestimated by conventional culture. In this study, real-time PCR was applied to detect Shigella infection in parallel with routine culture, to investigate the true burden of disease caused by Shigella spp. Methods: Rectal swab specimens of 39 Shigella culture positive and 298 Shigella culture negative patients from a population-based surveillance study were selected randomly. Real-time PCR targeting the invasion plasmid antigen H gene sequence (ipaH) was used to detect DNA sequences characteristic for Shigella spp. Results: ipaH were detected in 174 of 298 (58%) randomly selected Shigella culture negative specimens and in 38 of 39 (97%) Shigella culture positive specimens (p < 0.001). Among 10 variables, culture results was the strongest predictive factor (OR = 15.5; 95% CI: 2.0-119.0), followed by a clinical presentation of diarrhea with fever (OR = 2.8; 95% CI: 1.2-6.2), epidemic season (OR = 2.4; 95% CI: 1.4-4.3), and female gender (OR = 1.8; 95% CI: 1.1-3.0). Conclusion: The high detection rate of ipaH in culture negative specimens through use of real-time PCR suggests that earlier estimates of shigellosis burden measured by conventional culture may have underestimated the true disease burden. (C) 2010 The British Infection Association. Published by Elsevier Ltd. All rights reserved.
推荐引用方式
GB/T 7714：

Wang Song-Mei,Ma Jing-Chen,Hao Zhi-Yong, et al. Surveillance of shigellosis by real-time PCR suggests underestimation of shigellosis prevalence by culture-based methods in a population of rural China [J].JOURNAL OF INFECTION,2010,61(6):471-475.
APA：

Wang Song-Mei,Ma Jing-Chen,Hao Zhi-Yong,Zhang Zhi-Yong,&Xu Zhi-Yi.(2010).Surveillance of shigellosis by real-time PCR suggests underestimation of shigellosis prevalence by culture-based methods in a population of rural China .JOURNAL OF INFECTION,61(6):471-475.
MLA：

Wang Song-Mei, et al. "Surveillance of shigellosis by real-time PCR suggests underestimation of shigellosis prevalence by culture-based methods in a population of rural China" .JOURNAL OF INFECTION 61,6(2010):471-475.

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