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Liposomes and lipid disks traverse the BBB and BBTB as intact forms as revealed by two-step Forster resonance energy transfer imaging 期刊论文

编号：

10b17926-1fb9-4972-914d-6a29e14b0eb5
作者：

Dai, Tongcheng^[1,2,3,4,5];Jiang, Kuan(江宽)^[1,2,3,4]Lu, Weiyue(陆伟跃)*^{[1,2,3,4,5,6,7,8]}
语种：

English
期刊：

ACTA PHARMACEUTICA SINICA B ISSN：2211-3835 2018 年 8 卷 2 期 (261 - 271) ; MAR
收录：
关键词：

Liposomes Disks Intact form BBB BBTB FRET
摘要：

The blood brain barrier (BBB) and the blood brain tumor barrier (BBTB) prevent drug and nano-drug delivery systems from entering the brain. However, ligand-mediated nano-drug delivery systems have significantly enhanced the therapeutic treatment of glioma. In this study we investigated the mechanism especially the integrity of liposomes and lipid disks while traversing the BBB and BBTB both in vitro and in vivo. Fluorophores (DiO, DiI and DiD) were loaded into liposomes and lipid disks to form Forster resonance energy transfer (FRET) nano-drug delivery systems. Using brain capillary endothelial cells as a BBB model, we show that liposomes and disks are present in the cytoplasm as their intact forms and traverse the BBB with a ratio of 0.68 parts per thousand and 1.67 parts per thousand, respectively. Using human umbilical vein endothelial cells as BBTB model, liposomes and disks remained intact and traversed the BBTB with a ratio of 2.31 parts per thousand and 8.32 parts per thousand at 3 h. Ex vivo imaging and immunohistochemical results revealed that liposomes and disks could traverse the BBB and BBTB in vivo as intact forms. In conclusion, these observations explain in part the mechanism by which nano-drug delivery systems increase the therapeutic treatment of glioma (C) 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
推荐引用方式
GB/T 7714：

Dai Tongcheng,Jiang Kuan,Lu Weiyue, et al. Liposomes and lipid disks traverse the BBB and BBTB as intact forms as revealed by two-step Forster resonance energy transfer imaging [J].ACTA PHARMACEUTICA SINICA B,2018,8(2):261-271.
APA：

Dai Tongcheng,Jiang Kuan,Lu Weiyue.(2018).Liposomes and lipid disks traverse the BBB and BBTB as intact forms as revealed by two-step Forster resonance energy transfer imaging .ACTA PHARMACEUTICA SINICA B,8(2):261-271.
MLA：

Dai Tongcheng, et al. "Liposomes and lipid disks traverse the BBB and BBTB as intact forms as revealed by two-step Forster resonance energy transfer imaging" .ACTA PHARMACEUTICA SINICA B 8,2(2018):261-271.

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