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High-Throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array 期刊论文

编号：

1b5286a3-a8b5-426a-90bc-038bde39b198
作者：

Zhang, Ying(张英)#^[1,2]Zhao, Chunzhao^[3,4,5];Li, Li^[6];Hsu, ChuanChih^[7];Zhu, JianKang^[3,4,5];Iiuk, Anton*^[6]Tao, W. Andy^[7];
语种：

English
期刊：

ANALYTICAL CHEMISTRY ISSN：0003-2700 2018 年 90 卷 17 期 (10263 - 10270) ; SEP 4
收录：
摘要：

Protein phosphorylation is one of the most important and widespread molecular regulatory mechanisms that controls almost all aspects of cellular functions in animals and plants. Here, we introduce a novel chemically functionalized reverse phase phosphoprotein array (RP3A) to capture and measure phosphoproteomes. RP3A uses polyamidoamine (PAMAM) dendrimer immobilized with Ti(IV) ions to functionalize nitrocellulose membrane, facilitating specific chelation of phosphoproteins from complex protein samples on the array. Globular, water-soluble Ti(IV)-dendrimer allows the RP3A surface to be highly accessible to phosphoproteins multidimensionally, and the captured phosphoproteins were subsequently detected using the same validated antibodies as in regular reverse-phase protein arrays. The novel chemical strategy demonstrated superior specificity (1:10 000), high sensitivity (fg level), and good quantitative nature (R-2 = 0.99) for measuring phosphoproteins. We further applied quantitative phosphoproteomics followed by RP3A to validate the phosphorylation status of a panel of phosphoproteins in response to environmental stresses in Arabidopsis.
推荐引用方式
GB/T 7714：

Zhang Ying,Zhao Chunzhao,Li Li, et al. High-Throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array [J].ANALYTICAL CHEMISTRY,2018,90(17):10263-10270.
APA：

Zhang Ying,Zhao Chunzhao,Li Li,Hsu Chuan-Chih,&Tao W. Andy.(2018).High-Throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array .ANALYTICAL CHEMISTRY,90(17):10263-10270.
MLA：

Zhang Ying, et al. "High-Throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array" .ANALYTICAL CHEMISTRY 90,17(2018):10263-10270.
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