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Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge 期刊论文

编号：

1e54ce3b-e027-4ea4-a881-cd9235253f7b
作者：

Wang, JiaJian(王嘉健)#^[1]Liu, Chang#^[2]Shan, Kun#^[1,3,4]Liu, BaiHui^[5];Li, XiuMiao^[2];Zhang, ShuJie^[1,3,4];Zhou, RongMei^[1];Dong, Rui^[5];Yan, Biao*^[1,3,4]Sun, XingHuai(孙兴怀)*^[3,4,6,7,8]
语种：

English
期刊：

THERANOSTICS ISSN：1838-7640 2018 年 8 卷 12 期 (3408 - 3415)
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关键词：

circular RNA microRNA sponge glaucoma retinal neurodegeneration
摘要：

Glaucoma is a major cause of visual impairment characterized by progressive retinal neurodegeneration. Circular RNAs are a class of endogenous noncoding RNAs that regulate gene expression in eukaryotes. In this study, we investigated the role of cZNF609 in retinal neurodegeneration induced by glaucoma. Methods: qRT-PCR and Sanger sequencing were conducted to detect cZNF609 expression pattern during retinal neurodegeneration. Immunofluorescence staining was conducted to detect the effect of cZNF609 silencing on retinal neurodegeneration in vivo. MTT assay, Ki67 staining, and PI staining were conducted to detect the effect of cZNF609 silencing on retinal glial cells and RGC function in vitro. Bioinformatics analysis, RNA pull-down assays, and in vitro studies were conducted to reveal the mechanism of cZNF609-mediated retinal neurodegeneration. Results: cZNF609 expression was significantly up-regulated during retinal neurodegeneration. cZNF609 silencing reduced retinal reactive gliosis and glial cell activation, and facilitated RGC survival in vivo. cZNF609 silencing directly regulated Muller cell function but indirectly regulated RGC function in vitro. cZNF609 acted as an endogenous miR-615 sponge to sequester and inhibit miR-615 activity, which led to increased METRN. METRN overexpression could partially rescue cZNF609 silencing-mediated inhibitory effects on retinal glial cell proliferation. Conclusion: Intervention of cZNF609 expression is a promising therapeutic strategy for retinal neurodegeneration.
推荐引用方式
GB/T 7714：

Wang Jia-Jian,Liu Chang,Shan Kun, et al. Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge [J].THERANOSTICS,2018,8(12):3408-3415.
APA：

Wang Jia-Jian,Liu Chang,Shan Kun,Liu Bai-Hui,&Sun Xing-Huai.(2018).Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge .THERANOSTICS,8(12):3408-3415.
MLA：

Wang Jia-Jian, et al. "Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge" .THERANOSTICS 8,12(2018):3408-3415.
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