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Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications  期刊论文  

  • 编号:
    2050414f-4cdb-4168-8cec-d00449efce39
  • 作者:
    Jiang, Su(蒋苏)#[1]Liu, YaFeng[2,3];Wang, XiaoMin[4];Liu, KeFei[5];Zhang, DingHong[5,6];Li, YiDing[5,7];Yu, AiPing[1];Zhang, XiaoHui[7];Zhang, JiaYi(张嘉漪)[8,9]Xu, JianGuang(徐建光)[1,10]Gu, YuDong(顾玉东)[10]Xu, WenDong(徐文东)*[1,4,9]Zeng, ShaoQun[2,3];
  • 语种:
    English
  • 期刊:
    BIOMEDICAL OPTICS EXPRESS ISSN:2156-7085 2016 年 7 卷 9 期 (3478 - 3490) ; SEP 1
  • 收录:
  • 摘要:

    We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2 in vivo. Combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. Furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories. (C) 2016 Optical Society of America

  • 推荐引用方式
    GB/T 7714:
    Jiang Su,Liu Ya-Feng,Wang Xiao-Min, et al. Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications [J].BIOMEDICAL OPTICS EXPRESS,2016,7(9):3478-3490.
  • APA:
    Jiang Su,Liu Ya-Feng,Wang Xiao-Min,Liu Ke-Fei,&Zeng Shao-Qun.(2016).Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications .BIOMEDICAL OPTICS EXPRESS,7(9):3478-3490.
  • MLA:
    Jiang Su, et al. "Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications" .BIOMEDICAL OPTICS EXPRESS 7,9(2016):3478-3490.
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