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Generation of neural progenitor cells by chemical cocktails and hypoxia 期刊论文

编号：

20b0461b-7be8-4cf2-8894-3d7c3e84cd5a
作者：

Cheng Lin;Hu Wenxiang;Qiu Binlong;Zhao Jian;Yu Yongchun(禹永春); Guan Wuqiang;Wang Min;Yang Wuzhou;Pei Gang;
地址：

Cheng Lin, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai 200031, China. Hu Wenxiang, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai 200031, China. Qiu Binlong, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai 200031, China. Zhao Jian, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai 200031, China. Pei Gang, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai 200031, China. Yu Yongchun, Institute of Neurobiology, Institutes of Brain Science, Fudan University, State Key Laboratory of Medical Neurobiology, Shanghai 200032, China. Guan Wuqiang, Institute of Neurobiology, Institutes of Brain Science, Fudan University, State Key Laboratory of Medical Neurobiology, Shanghai 200032, China. Wang Min, Institute of Neurobiology, Institutes of Brain Science, Fudan University, State Key Laboratory of Medical Neurobiology, Shanghai 200032, China. Yang Wuzhou, Institute of Neurobiology, Institutes of Brain Science, Fudan University, State Key Laboratory of Medical Neurobiology, Shanghai 200032, China.
语种：

英文
期刊：

细胞研究 ISSN：1001-0602 2014 年 24 卷 6 期 (665 - 679) ; 2018-03-26
收录：
关键词：

lineage conversion neural progenitor cells chemical cocktails hypoxia
摘要：

Neural progenitor cells (NPCs) can be induced from somatic cells by defined factors. Here we report that NPCs can be generated from mouse embryonic fibroblasts by a chemical cocktail, namely VCR (V, VPA, an inhibitor of HDACs; C, CHIR99021, an inhibitor of GSK-3 kinases and R, Repsox, an inhibitor of TGF-beta pathways), under a physiological hypoxic condition. These chemical-induced NPCs (ciNPCs) resemble mouse brain-derived NPCs regarding their proliferative and self-renewing abilities, gene expression profiles, and multipotency for different neuroectodermal lineages in vitro and in vivo. Further experiments reveal that alternative cocktails with inhibitors of histone deacetylation, glycogen synthase kinase, and TGF-beta pathways show similar efficacies for ciNPC induction. Moreover, ciNPCs can also be induced from mouse tail-tip fibroblasts and human urinary cells with the same chemical cocktail VCR. Thus our study demonstrates that lineage-specific conversion of somatic cells to NPCs could be achieved by chemical cocktails without introducing exogenous factors.
推荐引用方式
GB/T 7714：

Cheng Lin,Hu Wenxiang,Qiu Binlong, et al. Generation of neural progenitor cells by chemical cocktails and hypoxia [J].细胞研究,2014,24(6):665-679.
APA：

Cheng Lin,Hu Wenxiang,Qiu Binlong,Zhao Jian,&Pei Gang.(2014).Generation of neural progenitor cells by chemical cocktails and hypoxia .细胞研究,24(6):665-679.
MLA：

Cheng Lin, et al. "Generation of neural progenitor cells by chemical cocktails and hypoxia" .细胞研究 24,6(2014):665-679.

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