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Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway 期刊论文

编号：

295aea58-a75e-403f-b255-6198301d364d
作者：

Zhu, Xiaopeng#^[1,2]Bian, Hua(卞华)*^[1,2]Wang, Liu^[1,2];Sun, Xiaoyang^[1,2];Xu, Xi(徐曦)^[1,2]Yan, Hongmei(颜红梅)^[1,2]Xia, Mingfeng(夏明锋)^[1,2]Chang, Xinxia(常薪霞)^[1,2]Lu, Yan(陆炎)^[1,2]Li, Yu^[3];Xia, Pu^[1,2];Li, Xiaoying^[1,2];Gao, Xin(高鑫)^[1,2]
语种：

英文
期刊：

FREE RADICAL BIOLOGY AND MEDICINE ISSN：0891-5849 2019 年 141 卷 (192 - 204) ; SEP
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关键词：

Berberine Nonalcoholic fatty liver disease NAFLD Stearyl-coenzyme A desaturase 1 SCD1 Sterol regulatory element-binding protein-1c SREBP-1c AMP-activated protein kinase AMPK
摘要：

Background: Berberine (BBR), a natural compound extracted from Chinese herb, has been shown to effectively attenuate nonalcoholic fatty liver disease (NAFLD) in clinic. However, the mechanism underlying the effect of BBR is not fully understood. Stearyl-coenzyme A desaturase 1 (SCD1) mediates lipid metabolism in liver. Therefore, we hypothesized that SCD1 mediated the beneficial effect of BBR on NAFLD.
Methods: The expression of SCD1 was measured in the liver of NAFLD patients and ob/ob mice. The effect of BBR on NAFLD was evaluated in C57BL/6 J mice on high fat diet (HFD). The effect of BBR was also investigated in HepG2 and AML12 cells exposed to high glucose and palmitic acid. Oil red O staining was performed to detect triglyceride (TG) level. Quantitative real-time polymerase chain reaction and Western blot were used to detect the messenger ribonucleic acid (mRNA) and protein expression of target genes. The activity of SCD1 promoter was measured by dual-luciferase reporter assay.
Results: The expression of SCD1 was increased in the liver of NAFLD patients and ob/ob mice. BBR reduced hepatic TG accumulation and decreased the expressions of hepatic SCD1 and other TG synthesis related genes both in vivo and in vitro. Knockdown of SCD1 expression mimicked the effect of BBR decreasing TG level in steatotic hepatocytes, whereas overexpression of SCD1 attenuated the effect of BBR. Mechanistically, BBR promoted the phosphorylation of AMP-activated protein kinase (AMPK) and sterol regulatory element-binding protein-1c (SREBP-1c) in HepG2 cells and the liver of HFD-fed mice. Activation of the AMPK-SREBP-lc pathway and sterol regulatory element (SRE) motif in SCD1 promoter (-920/-550) was responsible for the BBR-induced suppression of SCD1.
Conclusion: BBR reduces liver TG synthesis and attenuates hepatic steatosis through the activation of AMPK-SREBP-1c-SCD1 pathway.
推荐引用方式
GB/T 7714：

Zhu Xiaopeng,Bian Hua,Wang Liu, et al. Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway [J].FREE RADICAL BIOLOGY AND MEDICINE,2019,141:192-204.
APA：

Zhu Xiaopeng,Bian Hua,Wang Liu,Sun Xiaoyang,&Gao Xin.(2019).Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway .FREE RADICAL BIOLOGY AND MEDICINE,141:192-204.
MLA：

Zhu Xiaopeng, et al. "Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway" .FREE RADICAL BIOLOGY AND MEDICINE 141(2019):192-204.
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