Accumulating evidence suggests that noncoding RNAs (ncRNAs) have important regulatory functions. However, lacking of functional annotations for ncRNAs hampered us from carrying out the subsequent functional or predictive research. Here we dissected the expression profiles of 3,458 rat noncoding genes using rat bodymap RNA-sequencing data consisting of 11 solid organs over four developmental stages (juvenile, adolescent, adult and aged) from both sexes, and conducted a comprehensive analysis of differentially expressed noncoding genes (DEnGs) between various conditions. We then constructed a co-expression network between protein-coding and noncoding genes to infer biological functions of noncoding genes. Modules of interest were linked to online databases including DAVID for functional annotation and pathway analysis. Our results indicated that noncoding genes are functionally enriched through pathways similar to those of protein-coding genes. Terms about development of the immune system were enriched with genes from age-related modules, whereas terms about sexual reproduction were enriched with genes in sex-related modules. We also built connection networks on some significant modules to visualize the interactions and regulatory relationship between protein-coding and noncoding genes. Our study could improve our understanding and facilitate a deeper investigation on organ/age/sex-related regulatory events of noncoding genes, which may lead to a superior preclinical model for drug development and translational medicine.
[1]Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China.
[2]Fudan Univ, Sch Pharm, Ctr Pharmacogen, Shanghai 201203, Peoples R China.
[3]Fudan Univ, State Key Lab Genet Engn, Shanghai 201203, Peoples R China.
[4]Fudan Univ, Sch Life Sci, MOE Key Lab Contemporary Anthropol, Shanghai 201203, Peoples R China.
[5]Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China.
(8.0+极速模式)