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A new cultivation system for studying chemical effects on the lifespan of the fruit fly 期刊论文

编号：

2da998fa-d33f-4278-8385-88cd9e4ecf39
作者：

Li, Shuguang(厉曙光)#*^[1]Chen, Ke^[2];Li, Xin^[2];Zhang, Xinwen^[2];Liu, Shi V.^[3];
语种：

English
期刊：

EXPERIMENTAL GERONTOLOGY ISSN：0531-5565 2010 年 45 卷 2 期 (158 - 162) ; FEB
收录：
关键词：

Bottle Cultivation Fruit fly Drosophila melanogaster DEHP Lifespan Aging Mass balance Food-discharge separation Calorie/dietary-restriction
摘要：

A side-by-side comparison was made between a conventional vial system and a novel bottle system for cultivating flies and testing the effect of chemical exposure on the lifespan of the flies. While the two cultivation systems yielded very similar results for the effect of DEHP (di[2-ethylhexyl] phthalate) on reducing the lifespan of fruit fly Drosophila melanogaster, the new bottle system has many advantages over the conventional vial system. The bottle system allowed long-term cultivation of flies in the same bottle and thus eliminated the need for transferring of flies between vials. Foods/nutrients were provided as fresh moisture medium coated on a glass slide vertically hanged in the center of the bottle. Fly discharges and dead flies were collected onto a draw horizontally inserted into the bottom of the bottle. These features have resulted in great convenience for cultivating flies and reduced labor and media cost. The effective separation of food from discharge may allow accurate mass balance measurement and thus yield more definitive observations for understanding the actual role of calorie restriction (CR) or dietary-restriction (DR) in fly metabolism and longevity. (C) 2009 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Li Shuguang,Chen Ke,Li Xin, et al. A new cultivation system for studying chemical effects on the lifespan of the fruit fly [J].EXPERIMENTAL GERONTOLOGY,2010,45(2):158-162.
APA：

Li Shuguang,Chen Ke,Li Xin,Zhang Xinwen,&Liu Shi V..(2010).A new cultivation system for studying chemical effects on the lifespan of the fruit fly .EXPERIMENTAL GERONTOLOGY,45(2):158-162.
MLA：

Li Shuguang, et al. "A new cultivation system for studying chemical effects on the lifespan of the fruit fly" .EXPERIMENTAL GERONTOLOGY 45,2(2010):158-162.

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