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High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts 期刊论文

编号：

30756647-9c4a-4d62-8081-d58e6c50d252
作者：

Kang, Yu^[2];Yu, Wenbo^[3];Sun, Qinwen^[3];Zhang, Xiaoyan^[2];Jiang, Wei^[2];Wu, Chaoqun^[3];Chen, Chunmei^[3];Gu, Jianren^[5];Zheng, Yufang^[4];Xu, Congjian(徐丛剑)*^[1,2,6]
语种：

English
期刊：

ONCOLOGY REPORTS ISSN：1021-335X 2010 年 24 卷 4 期 (897 - 907) ; OCT
收录：
关键词：

piggyBac transposon herpes simplex virus thymidine kinase non-viral vector gene delivery cervical cancer
摘要：

The piggyBac (PB) transposon is a recently identified, active and flexible transgene vector, combining the advantages of non-viral gene delivery with genomic integration and persistent transgene expression. In this study, we utilized the PB transposon to carry the herpes simplex thymidine kinase (HSV-tk) and red fluorescent protein (mRFP1) reporter genes into the He La cervical cancer cell line or tumor xenografts of cervical cancer. Our data showed that HSV-tk and mRFP1 were expressed in He La cells and tumor xenografts three weeks after intratumoral injection. The mRNA and protein levels of HSV-tk and mRFP1 were increased by using the PB transposon vector. Our system also demonstrated that sensitivity of transfected He La cells to the pro-drug ganciclovir (GCV) was enhanced in vitro and in vivo. Furthermore, our data indicated that the enhanced transgenic therapeutic effect was strongly associated with high-level transgene expression mediated by the PB transposon. Our results suggest that applying the PB transposon in HSV-tk gene delivery and GCV treatment is a promising gene therapy strategy in the treatment of cervical cancer.
推荐引用方式
GB/T 7714：

Kang Yu,Yu Wenbo,Sun Qinwen, et al. High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts [J].ONCOLOGY REPORTS,2010,24(4):897-907.
APA：

Kang Yu,Yu Wenbo,Sun Qinwen,Zhang Xiaoyan,&Xu Congjian.(2010).High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts .ONCOLOGY REPORTS,24(4):897-907.
MLA：

Kang Yu, et al. "High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts" .ONCOLOGY REPORTS 24,4(2010):897-907.

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