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Distinctive Drug-resistant Mutation Profiles and Interpretations of HIV-1 Proviral DNA Revealed by Deep Sequencing in Reverse Transcriptase 期刊论文

编号：

33152a68-8db1-4de3-aed9-dfbabeec104e
作者：

Yin Qian Qian^[1];Li Zhen Peng^[1,2];Zhao Hai^[1];Pan Dong^[1];Wang Yan^[1];Xu Wei Si^[1];Xing Hui^[1];Feng Yi^[1];Jiang Shi Bo(姜世勃)^[3,4,5]Shao Yi Ming^[1];Ma Li Ying^[1];
语种：

English
期刊：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES ISSN：0895-3988 2016 年 29 卷 4 期 (239 - 247) ; APR
收录：
关键词：

HIV-1 drug-resistant mutation Drug-resistance interpretation Proviral DNA Viral RNA Deep sequencing
摘要：

Objective To investigate distinctive features in drug-resistant mutations (DRMs) and interpretations for reverse transcriptase inhibitors (RTIs) between proviral DNA and paired viral RNA in HIV-1-infected patients. Methods Forty-three HIV-1-infected individuals receiving first-line antiretroviral therapy were recruited to participate in a multicenter AIDS Cohort Study in Anhui and Henan Provinces in China in 2004. Drug resistance genotyping was performed by bulk sequencing and deep sequencing on the plasma and whole blood of 77 samples, respectively. Drug-resistance interpretation was compared between viral RNA and paired proviral DNA. Results Compared with bulk sequencing, deep sequencing could detect more DRMs and samples with DRMs in both viral RNA and proviral DNA. The mutations M184I and M230I were more prevalent in proviral DNA than in viral RNA (Fisher's exact test, P<0.05). Considering 'majority resistant variants', 15 samples (19.48%) showed differences in drug resistance interpretation between viral RNA and proviral DNA, and 5 of these samples with different DRMs between proviral DNA and paired viral RNA showed a higher level of drug resistance to the first-line drugs. Considering 'minority resistant variants', 22 samples (28.57%) were associated with a higher level of drug resistance to the tested RTIs for proviral DNA when compared with paired viral RNA. Conclusion Compared with viral RNA, the distinctive information of DRMs and drug resistance interpretations for proviral DNA could be obtained by deep sequencing, which could provide more detailed and precise information for drug resistance monitoring and the rational design of optimal antiretroviral therapy regimens.
推荐引用方式
GB/T 7714：

Yin Qian Qian,Li Zhen Peng,Zhao Hai, et al. Distinctive Drug-resistant Mutation Profiles and Interpretations of HIV-1 Proviral DNA Revealed by Deep Sequencing in Reverse Transcriptase [J].BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2016,29(4):239-247.
APA：

Yin Qian Qian,Li Zhen Peng,Zhao Hai,Pan Dong,&Ma Li Ying.(2016).Distinctive Drug-resistant Mutation Profiles and Interpretations of HIV-1 Proviral DNA Revealed by Deep Sequencing in Reverse Transcriptase .BIOMEDICAL AND ENVIRONMENTAL SCIENCES,29(4):239-247.
MLA：

Yin Qian Qian, et al. "Distinctive Drug-resistant Mutation Profiles and Interpretations of HIV-1 Proviral DNA Revealed by Deep Sequencing in Reverse Transcriptase" .BIOMEDICAL AND ENVIRONMENTAL SCIENCES 29,4(2016):239-247.

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