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Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells 期刊论文

编号：

33b8615a-811d-4b2b-9428-99f7d83067d9
作者：

Yan, Yuan(闫媛)^[1,2,3]Yin, Peipei(殷培培)^[1,2]Gong, Hui^[1,2];Xue, Yuanyuan^[1,2];Zhang, Guoping^[1,2];Fang, Bo^[1,2];Chen, Zhidan^[1,2];Li, Yang^[1,2];Yang, Chunjie^[1,2];Huang, Zheyong^[1,2];Yang, Xiangdong^[1,2];Ge, Junbo(葛均波)^[1,2]Zou, Yunzeng(邹云增)^[1,2]
语种：

English
期刊：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY ISSN：1015-8987 2016 年 38 卷 1 期 (340 - 350)
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关键词：

Nap1l1 iPS Proliferation Apoptosis Cell cycle
摘要：

Background/Aims: To investigate whether nucleosome assembly protein 1-like 1 (Nap1l1) regulates the proliferation of induced pluripotent stem cells (iPSC) and the potential mechanisms. Methods: Nap1l1-knockdown-iPSC and Nap1l1-overexpression-iPSC were constructed by transfection of lentiviral particles. The proliferation of iPSC was detected by MTT analysis, and cell cycle was analyzed by flow cytometry. Results: Nap1l1 overexpression promoted iPSC proliferation and induced G2/M transition compared to their control iPSC while Nap1l1-knockdown-iPSC dramatically displayed the reduced proliferation and accumulated G2/M phase cells. Further analysis showed that Nap1l1 overexpression in iPSC increased the expression of cyclin B1, downregulated the expression of p21 and p27, while knockdown of Nap1l1 showed the opposite effects. In addition, overexpression of Nap1l1 promoted the phosphorylation of AKT and ERK in iPSC, while knockdown of Nap1l1 inhibited the effects. However, these effects displayed in Nap1l1-overexpression-iPSC were greatly suppressed by the inhibition of AKT or ERK signaling. Conclusions: The results indicate that Nap1l1 promotes the proliferation of iPSC attributable to G2/M transition caused by downregulation of p27 and p21, and upregulation of cyclin B1, the activation of AKT or ERK is involved in the process. The present study has revealed a novel molecular mechanism involved in the proliferation of iPSC. (C) 2016 The Author(s) Published by S. Karger AG, Basel
推荐引用方式
GB/T 7714：

Yan Yuan,Yin Peipei,Gong Hui, et al. Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells [J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2016,38(1):340-350.
APA：

Yan Yuan,Yin Peipei,Gong Hui,Xue Yuanyuan,&Zou Yunzeng.(2016).Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,38(1):340-350.
MLA：

Yan Yuan, et al. "Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells" .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 38,1(2016):340-350.

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