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Glycosylation patterns and PHA-E-associated glycoprotein profiling associated with early hepatic encephalopathy in Chinese hepatocellular carcinoma patients 期刊论文

编号：

35014032-204f-4d66-b2c4-c1492fdf7747
作者：

Liu, TianHua^[1,2];Liu, DengHe^[3];Mo, CuiJu^[3];Sun, Lu(孙璐)^[2]Liu, XiaoXia(刘晓霞)^[4]Li, Wei(李威)^[2]Zhang, Shu^[1];Liu, YinKun(刘银坤)^[1,2]Guo, Kun^[1];
语种：

English
期刊：

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH ISSN：1943-8141 2016 年 8 卷 10 期 (4250 - +)
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关键词：

Hepatocellular carcinoma hepatic encephalopathy glycoproteomics lectin microarray lectin-affinity chromatography LC-MS/MS
摘要：

Hepatic encephalopathy (HE) as a severe neuropsychiatric complication is commonly present in the end stage of Hepatocellular Carcinoma (HCC). However, widely accepted biomarkers for diagnosing early HE are still absent. Here, we screened glycosylation patterns of serum proteins from Chinese HCC patients with or without early HE by lectin microarray. Then, phaseolus vulgaris erythroagglutinin (PHA-E) as a lectin binding with bisecting GlcNAc structure which was significantly decreased in sera from Chinese HCC patients with early HE, was chosen to perform lectin affinity chromatography, following by in-gel digestion, Mass Spectrometry (MS) analysis and bioinformatics analysis. Here we found, 13 lectins showed statistically significant reduction suggesting GalNAc, terminal alpha-1,3 Man, bisecting GlcNAc, (GlcNAc)n, O-GlcNAc, Neu5Ac, tetra-antennary complex-type N-glycan and GalNAc alpha/beta 1-3/6 Gal were decreased in serum glycoproteins from Chinese HCC patients with early HE. Furthermore, a total of 141 PHA-E-associated glycoproteins were identified in MS, of which 12 serum glycoproteins only in Chinese HCC patients without early HE and 26 serum glycoproteins only in Chinese HCC patients with early HE. In addition, bioinformatics analysis revealed the PHA-E-associated serum glycoproteins only in Chinese HCC patients with early HE might be related to early HE occurrence through p38 MAPK signaling pathway and MAPK/ERK signaling pathway. Collectively, this was the first glycomics study of serum proteins in HCC patients with early HE and it could provide a database for discovering and developing serum biomarkers to identify and predict early HE in HCC patients.
推荐引用方式
GB/T 7714：

Liu Tian-Hua,Liu Deng-He,Mo Cui-Ju, et al. Glycosylation patterns and PHA-E-associated glycoprotein profiling associated with early hepatic encephalopathy in Chinese hepatocellular carcinoma patients [J].AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2016,8(10):4250-+.
APA：

Liu Tian-Hua,Liu Deng-He,Mo Cui-Ju,Sun Lu,&Guo Kun.(2016).Glycosylation patterns and PHA-E-associated glycoprotein profiling associated with early hepatic encephalopathy in Chinese hepatocellular carcinoma patients .AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,8(10):4250-+.
MLA：

Liu Tian-Hua, et al. "Glycosylation patterns and PHA-E-associated glycoprotein profiling associated with early hepatic encephalopathy in Chinese hepatocellular carcinoma patients" .AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 8,10(2016):4250-+.

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