In the current study, it was aimed to evaluate the changes in myelinated and unmyelinated nerve fibers in retinal ischemia-reperfusion injuries caused by acute ocular hypertension and to determine the sequence of these changes. Adult healthy New Zealand white rabbits were randomized to the hemodynamic group [n=12; used to determine the optimal intraocular pressure (IOP) for the subsequent experiments] and the hypertension group (n=6; 70-mmHg hypertension induced in one eye). IOP was adjusted using a cannula and saline. Doppler ultrasound was used to measure the velocity of the optic artery under different intraocular pressures. Immunohistochemistry for myelin basic protein (MBP) was performed. Apoptosis of retinal cells was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay. Electron microscopy was used to investigate the changes in myelinated and unmyelinated nerve fibers. IOP of the hypertension eyes was maintained at 70.2 +/- 1.0 mmHg, while IOP of control eyes was 7-14 mmHg. Doppler ultrasound demonstrated an obvious decline of peak systolic velocity and an increase of resistance index of retinal bloodstream under a 70-mmHg IOP. MBP immunohistochemistry and electron microscopy demonstrated obvious injuries to the myelin fibers. TUNEL indicated a significantly higher apoptosis rate in the hypertension eyes compared with control eyes. The apoptosis rate of retinal ganglion cells and bipolar cells in unmyelinated an IOP of 70 mmHg led to incomplete retinal ischemia but was the threshold for retinal ischemia, leading to obvious injuries to the myelin fibers.
[1]Shanghai Peace Eye Hosp, Dept Ophthalmol, Shanghai 200437, Peoples R China.
[2]Fudan Univ, Eye & ENT Hosp, Dept Ophthalmol, Shanghai 200031, Peoples R China.
[3]Univ Western Australia, Ctr Ophthalmol & Visual Sci, Perth, WA 6009, Australia.
[4]Shanghai Jiao Tong Univ, Sch Med, Shanghai Ninth Peoples Hosp, Dept Ophthalmol, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China.
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