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Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2 期刊论文

编号：

35dd1f46-8ebb-4f5a-9c8b-90ec5102d306
作者：

Kong, DS; Ma, DA; Bai, H; Guo, HS; Cai, X; Mo, W; Tang, QQ; Song, HY
语种：

English
期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSN：0006-291X 2004 年 324 卷 4 期 (1179 - 1185) ; NOV 26
收录：
关键词：

human tissue factor pathway inhibitor-2 kunitz domain Pichia pastoris protein expression purification trypsin plasmin
摘要：

Human tissue factor pathway inhibitor-2 (hTFPI-2) has three kunitz domains whose structure and function are unclear. We expressed the first kunitz domain of hTFPI-2 (hTFPI-2/KD1) as functional form using Pichia pastoris and investigated its characterization. In the experiment, hTFPI-2/KD1 can inhibit the plasmin and trypsin activity and the Ki of hTFPI-2/KD1 towards plasmin (30 nM) and trypsin (50 nM) was determined as 10 and 7 nM by chromogenic assay, respectively. hTFPI-2/KD1 can also inhibit MMP-2 and MMP-9 in zymography assay. Furthermore, the inhibition of hTFPI-2/KD1 to the Matrigel invasion by HT-1080 is also described. This study provides a method to produce hTFPI-2/KD1 efficiently and some insights into the structure and function of hTFPI-2/KD1. (C) 2004 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Kong DS,Ma DA,Bai H, et al. Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2 [J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,324(4):1179-1185.
APA：

Kong DS,Ma DA,Bai H,Guo HS,&Song HY.(2004).Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2 .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,324(4):1179-1185.
MLA：

Kong DS, et al. "Expression and characterization of the first kunitz domain of human tissue factor pathway inhibitor-2" .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 324,4(2004):1179-1185.

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