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MS4A8B Promotes Cell Proliferation in Prostate Cancer 期刊论文

编号：

36eac86d-8493-44cd-9b2a-019ddbd5c988
作者：

Ye, Lin^[1,2,3];Yao, XuDong(姚旭东)^[1,2,3]Wan, FangNing^[1,3];Qu, YuanYuan(瞿元元)^[1,3]Liu, ZhiYu^[4];Shen, XuXia(沈旭霞)^[3,5]Li, Sheng^[6];Liu, XiaoJun(刘晓军)^[1,3]Yue, Feng^[7];Wang, Na^[3,8];Dai, Bo(戴波)^[1,3]Ye, DingWei(叶定伟)*^[1,3]
语种：

English
期刊：

PROSTATE ISSN：0270-4137 2014 年 74 卷 9 期 (911 - 922) ; JUN
收录：
关键词：

prostate cancer MS4A8B proliferation cell cycle
摘要：

BACKGROUND. Prostate cancer cells must maintain or achieve the further ability of proliferation during the progression. The molecular mechanisms, however, remain poorly understood. We identified a novel oncogene, termed membrane-spanning 4-domains, subfamily A, member 8B (MS4A8B), over-expressed in prostate cancer. METHODS. We firstly detected MS4A8B mRNA in 13 types of paired human normal and cancer tissues by real-time polymerase chain reaction (RT-PCR). In 140 clinically localized prostate cancer samples from radical prostatectomy, immunohistochemical staining was performed to study MS4A8B and PCNA protein level as an index of proliferative activity, TUNEL staining as an index of apoptosis. As MS4A8B RNAi and cDNA transfection technologies were used, the effect of MS4A8B on cellular vitality was determined in vitro and in vivo. RESULTS. MS4A8B mRNA was over-expressed specifically in prostate cancer. Positive ratios of MS4A8B protein expression were 1.94%, 5.92%, and 62.8% in benign, HPIN and prostate cancer, respectively. Moreover, MS4A8B was positively associated with Gleason score, the proliferation index. In vitro, MS4A8B knockdown resulted in G(1)-S cell cycle arrest and descended vitality, MS4A8B over-expression with accelerated S phase entry, elevated vitality in prostate cancer cells. Moreover, it was also found that expression of MS4A8B led to changes of Cyclin D-1, Cyclin E-1 and PCNA. LNCaP cells transfected with sh-MS4A8B lentivirus particles grew more slowly when subcutaneously injected into the flanks of nude mice. CONCLUSIONS. We conclude that the expression of MS4A8B expression promotes cell proliferation and plays an important role in carcinogenesis and progression of prostate cancer. (c) 2014 Wiley Periodicals, Inc.
推荐引用方式
GB/T 7714：

Ye Lin,Yao Xu-Dong,Wan Fang-Ning, et al. MS4A8B Promotes Cell Proliferation in Prostate Cancer [J].PROSTATE,2014,74(9):911-922.
APA：

Ye Lin,Yao Xu-Dong,Wan Fang-Ning,Qu Yuan-Yuan,&Ye Ding-Wei.(2014).MS4A8B Promotes Cell Proliferation in Prostate Cancer .PROSTATE,74(9):911-922.
MLA：

Ye Lin, et al. "MS4A8B Promotes Cell Proliferation in Prostate Cancer" .PROSTATE 74,9(2014):911-922.

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