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Single-Cell Approach to Influenza Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection 期刊论文

编号：

382b1622-bd0c-4791-833c-230c43dd252a
作者：

Sant, Sneha^[1];Grzelak, Ludiyine^[1,2];Wang, Zhongfang^[1];Pizzollal, Angela^[1];Koutsakos, Manbs^[1];Crowe, Jane^[3];Loudoyaris, Thomas^[4];Mannering, Stuart I.^[4];Westall, Glen P.^[5];Wakiml, Linda M.^[1];Rossjohn, Jamie^[6,7,8];Gras, Stephanie^[6,7];Richards, Michael^[9];Xu, Jianqing(徐建青)^[10]Thomas, Paul G.^[11];Loh, Liyen^[1];Nguyen, Thi H.^[1];Kedzierska, Katherine^[1];
语种：

English
期刊：

FRONTIERS IN IMMUNOLOGY ISSN：1664-3224 2018 年 9 卷 ; JUN 27
收录：
关键词：

T cell receptor repertoire influenza A virus human T lymphocytes CD81 T cells aging tissues
摘要：

CD8(+) T cells recognizing antigenic peptides derived from conserved internal viral proteins confer broad protection against distinct influenza viruses. As memory CD8(+) T cells change throughout the human lifetime and across tissue compartments, we investigated how T cell receptor (TCR) composition and diversity relate to memory CD8(+) T cells across anatomical sites and immunological phases of human life. We used ex vivo peptide-HLA tetramer magnetic enrichment, single-cell multiplex RT-PCR for both the TCR-alpha (TCR alpha) and TCR-beta (TCR beta) chains, and new TCRdist and grouping of lymphocyte interactions by paratope hotspots (GLIPH) algorithms to compare TCRs directed against the most prominent human influenza epitope, HLA-A*02:01-M1(58-66) (A2(+)M1(58)). We dissected memory TCR repertoires directed toward A2(+)M1(58) CD8(+) T cells within human tissues and compared them to human peripheral blood of young and elderly adults. Furthermore, we compared these memory CD8(+) T cell repertoires to A2(+)M1(58) CD8(+) TCRs during acute influenza disease in patients hospitalized with avian A/H7N9 virus. Our study provides the first ex vivo comparative analysis of paired antigen-specific TCR-alpha/beta clonotypes across different tissues and peripheral blood across different age groups. We show that human A2(+)M1(58) CD8(+) T cells can be readily detected in human lungs, spleens, and lymph nodes, and that tissue A2(+)M1(58) TCR alpha beta repertoires reflect A2(+)M1(58) TCR alpha beta clonotypes derived from peripheral blood in healthy adults and influenza-infected patients. A2(+)M1(58) TCR alpha beta repertoires displayed distinct features only in elderly adults, with large private TCR alpha beta clonotypes replacing the prominent and public TRBV19/TRAV27 TCRs. Our study provides novel findings on influenza-specific TCR alpha beta repertoires within human tissues, raises the question of how we can prevent the loss of optimal TCR alpha beta signatures with aging, and provides important insights into the rational design of T cell-mediated vaccines and immunotherapies.
推荐引用方式
GB/T 7714：

Sant Sneha,Grzelak Ludiyine,Wang Zhongfang, et al. Single-Cell Approach to Influenza Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection [J].FRONTIERS IN IMMUNOLOGY,2018,9.
APA：

Sant Sneha,Grzelak Ludiyine,Wang Zhongfang,Pizzollal Angela,&Kedzierska Katherine.(2018).Single-Cell Approach to Influenza Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection .FRONTIERS IN IMMUNOLOGY,9.
MLA：

Sant Sneha, et al. "Single-Cell Approach to Influenza Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection" .FRONTIERS IN IMMUNOLOGY 9(2018).

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