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First Report of a Clinical, Multidrug-Resistant Enterobacteriaceae Isolate Coharboring Fosfomycin Resistance Gene fosA3 and Carbapenemase Gene bla(KPC-2) on the Same Transposon, Tn1721 期刊论文

编号：

3a4420dc-a19c-4201-9040-e7fb32d39911
作者：

Li, Gang(李刚)^[1,2]Zhang, Ying(张颖)^[2]Bi, Dexi^[3,4];Shen, Pinghua^[2];Ai, Fuqi(艾芙琪)^[2]Liu, Hong^[2];Tian, Yueru(田月如)^[2]Ma, Yiming^[2];Wang, Bei^[2];Rajakumar, Kumar^[5,6];Ou, HongYu^[3,4];Jiang, Xiaofei(蒋晓飞)^[2]
语种：

English
期刊：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY ISSN：0066-4804 2015 年 59 卷 1 期 (338 - 343) ; JAN
收录：
摘要：

In order to understand the genetic background and dissemination mechanism of carbapenem resistance and fosfomycin resistance in Enterobacteriaceae isolates, we studied a clinical Escherichia coli strain HS102707 isolate and an Enterobacter aerogenes strain HS112625 isolate, both of which were resistant to carbapenem and fosfomycin and positive for the bla(KPC-2) and fosA3 genes. In addition, a clinical Klebsiella pneumoniae strain HS092839 isolate which was resistant to carbapenem was also studied. A 70-kb plasmid was successfully transferred to recipient E. coli J53 by a conjugation test. PCR and Southern blot analysis showed that bla(KPC-2) was located on this plasmid. The complete sequence of pHS102707 showed that this plasmid belongs to the P11 subfamily (IncP1) and has a replication gene, several plasmid-stable genes, an intact type IV secretion system gene cluster, and a composite transposon Tn1721-Tn3 that harbored bla(KPC-2). Interestingly, a composite IS26 transposon carrying fosA3 was inserted in the Tn1721-tnpA gene in pHS102707 and pHS112625, leading to the disruption of Tn1721-tnpA and the deletion of Tn1721-tnpR. However, only IS26 with a truncated Tn21-tnpR was inserted in pHS092839 at the same position. To our knowledge, this is the first report of fosA3 and bla(KPC-2) colocated in the same Tn1721-Tn3-like composite transposon on a novel IncP group plasmid.
推荐引用方式
GB/T 7714：

Li Gang,Zhang Ying,Bi Dexi, et al. First Report of a Clinical, Multidrug-Resistant Enterobacteriaceae Isolate Coharboring Fosfomycin Resistance Gene fosA3 and Carbapenemase Gene bla(KPC-2) on the Same Transposon, Tn1721 [J].ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2015,59(1):338-343.
APA：

Li Gang,Zhang Ying,Bi Dexi,Shen Pinghua,&Jiang Xiaofei.(2015).First Report of a Clinical, Multidrug-Resistant Enterobacteriaceae Isolate Coharboring Fosfomycin Resistance Gene fosA3 and Carbapenemase Gene bla(KPC-2) on the Same Transposon, Tn1721 .ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,59(1):338-343.
MLA：

Li Gang, et al. "First Report of a Clinical, Multidrug-Resistant Enterobacteriaceae Isolate Coharboring Fosfomycin Resistance Gene fosA3 and Carbapenemase Gene bla(KPC-2) on the Same Transposon, Tn1721" .ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 59,1(2015):338-343.

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