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Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice 期刊论文

编号：

3b327788-fc9d-4349-8986-5d88f1377670
作者：

Li, Meili[0]^[1] Fei, Zhaoliang[1]^[2] Ren, Jianke[2]^[3] Sun, Ruilin[3]^[3] Liu, Zhihui[4]^[4] Sheng, Zhejin[5]^[5] Wang, Long[6]^[3] Sun, Xia[7]^[3] Jun Yu, Jun[8]^[6] Wang, Zhugang[9]^[3] Fei, Jian[10]^[3]
地址：

[1]Chengdu Medical College, Research Center,Chengdu,China
[2]Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences, Shanghai Key Laboratory of Molecular Andrology,Shanghai,China
[3]Shanghai Research Center for Model Organisms,Shanghai,China
[4]Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences, Laboratory of Molecular Cell Biology,Shanghai,China
[5]Fudan University School of Life Sciences,Shanghai,China
[6]University of Strathclyde, Strathclyde Institute of Pharmacy and Biomedical Sciences,Glasgow,United Kingdom
语种：

英文
期刊：

BMC Immunology ISSN：1471-2172 2008 年 9 卷
收录：
摘要：

Background: Interleukin 1 beta (IL-1β) plays an important role in a number of chronic and acute inflammatory diseases. To understand the role of IL-1β in disease processes and develop an in vivo screening system for anti-inflammatory drugs, a transgenic mouse line was generated which incorporated the transgene firefly luciferase gene driven by a 4.5-kb fragment of the human IL-1β gene promoter. Luciferase gene expression was monitored in live mice under anesthesia using bioluminescence imaging in a number of inflammatory disease models. Results: In a LPS-induced sepsis model, dramatic increase in luciferase activity was observed in the mice. This transgene induction was time dependent and correlated with an increase of endogenous IL-1β mRNA and pro-IL-1β protein levels in the mice. In a zymosan-induced arthritis model and an oxazolone-induced skin hypersensitivity reaction model, luciferase expression was locally induced in the zymosan injected knee joint and in the ear with oxazolone application, respectively. Dexamethasone suppressed the expression of luciferase gene both in the acute sepsis model and in the acute arthritis model. Conclusion: Our data suggest that the transgenic mice model could be used to study transcriptional regulation of the IL-1β gene expression in the inflammatory process and evaluation the effect of anti-inflammatory drug in vivo. © 2008 Li et al; licensee BioMed Central Ltd.
推荐引用方式
GB/T 7714：

Li Meili/35201335000[0],Fei Zhaoliang/25625976000[1],Ren Jianke/23668680100[2], et al. Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice [J].BMC Immunology,2008,9.
APA：

Li Meili/35201335000[0],Fei Zhaoliang/25625976000[1],Ren Jianke/23668680100[2],Sun Ruilin/19337839100[3],&Fei Jian/7007033322[10].(2008).Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice .BMC Immunology,9.
MLA：

Li Meili/35201335000[0], et al. "Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice" .BMC Immunology 9(2008).

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