首页 / 院系成果 / 成果详情页

Phosphorescence Monitoring of Hypoxic Microenvironment in Solid-Tumors to Evaluate Chemotherapeutic Effects Using the Hypoxia-Sensitive Iridium (III) Coordination Compound 期刊论文

编号：

3c0dfc92-7544-4b23-b6d2-4bc92781a608
作者：

Zeng, Yun^[1];Liu, Yang^[2,3];Shang, Jin^[4];Ma, Jingwen^[1];Wang, Rong^[4];Deng, Lei^[4];Guo, Youmin^[4];Zhong, Fan(钟凡)^[2,3]Bai, Mingfeng^[5];Zhang, Shaojuan^[4,5];Wu, Daocheng^[1];
语种：

English
期刊：

PLOS ONE ISSN：1932-6203 2015 年 10 卷 3 期 ; MAR 18
收录：
摘要：

Objectives To utilize phosphorescence to monitor hypoxic microenvironment in solid-tumors and investigate cancer chemotherapeutic effects in vivo. Methods A hypoxia-sensitive probe named BTP was used to monitor hypoxic microenvironment in solid-tumors. The low-dose metronomic treatment with cisplatin was used in anti-angiogenetic chemotherapeutic programs. The phosphorescence properties of BTP were detected by a spectrofluorometer. BTP cytotoxicity utilized cell necrosis and apoptosis, which were evaluated by trypan blue dye exclusion and Hoechst33342 plus propidium iodide assays. Tumor-bearing mouse models of colon adenocarcinoma were used for tumor imaging in vivo. Monitoring of the hypoxic microenvironment in tumors was performed with a Maestro 2 fluorescence imaging system. Tumor tissues in each group were harvested regularly and treated with pathological hematoxylin and eosin and immunohistochemical staining to confirm imaging results. Results BTP did not feature obvious cytotoxicity for cells, and tumor growth in low-dose metronomic cisplatin treated mice was significantly inhibited by chemotherapy. Hypoxic levels significantly increased due to cisplatin, as proven by the expression level of related proteins. Phosphorescence intensity in the tumors of mice in the cisplatin group was stronger and showed higher contrast than that in tumors of saline treated mice. Conclusions We develop a useful phosphorescence method to evaluate the chemotherapeutic effects of cisplatin. The proposed method shows potential as a phosphorescence imaging approach for evaluating chemotherapeutic effects in vivo, especially anti-angiogenesis.
推荐引用方式
GB/T 7714：

Zeng Yun,Liu Yang,Shang Jin, et al. Phosphorescence Monitoring of Hypoxic Microenvironment in Solid-Tumors to Evaluate Chemotherapeutic Effects Using the Hypoxia-Sensitive Iridium (III) Coordination Compound [J].PLOS ONE,2015,10(3).
APA：

Zeng Yun,Liu Yang,Shang Jin,Ma Jingwen,&Wu Daocheng.(2015).Phosphorescence Monitoring of Hypoxic Microenvironment in Solid-Tumors to Evaluate Chemotherapeutic Effects Using the Hypoxia-Sensitive Iridium (III) Coordination Compound .PLOS ONE,10(3).
MLA：

Zeng Yun, et al. "Phosphorescence Monitoring of Hypoxic Microenvironment in Solid-Tumors to Evaluate Chemotherapeutic Effects Using the Hypoxia-Sensitive Iridium (III) Coordination Compound" .PLOS ONE 10,3(2015).

浏览次数：3  下载次数：0

分享到：

浏览次数：3

下载次数：0

打印次数：0