Optical imaging provides an indispensable way to locate tumors in their early stages with high sensitivity and signal to background ratio. A heptamethine cyanine based fluorophore that emits both single photon near-infrared fluorescence and two-photon deep red fluorescence under physiological conditions was developed. Linear and nonlinear photophysical properties of this fluorophore were investigated and it demonstrated the capability to label lysosomes in cancer cells. The advantages of this fluorophore, including tolerable cytotoxicity, high fluorescence quantum yield, and the ability to emit both near-infrared single photon fluorescence and deep red two photon fluorescence in aqueous solution, give it potential to be used in intra-operatively optical image-guided tumor excision followed by two-photon fluorescence microscopy biopsy analysis after a single administration.
[1]Southeast Univ, Sch Med, Zhongda Hosp, Jiangsu Key Lab Mol & Funct Imaging,Dept Radiol, Nanjing 210009, Jiangsu, Peoples R China.
[2]Fudan Univ, Sch Pharm, Minist Educ, Key Lab Smart Drug Delivery, Shanghai 201203, Peoples R China.
[3]Fudan Univ, Sch Pharm, PLA, Shanghai 201203, Peoples R China.
[4]Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China.
[5]Hong Kong Baptist Univ, Dept Phys, Ctr Adv Luminescence Mat, Kowloon, Hong Kong, Peoples R China.
[6]Hong Kong Polytech Univ, Shenzhen Res Inst, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China.
(8.0+极速模式)