Hepatocellular carcinoma is one of the most mortal and prevalent cancers with increasing incidence worldwide. Elucidating genetic driver genes for prognosis and palindromia of hepatocellular carcinoma helps managing clinical decisions for patients. In this study, the high-throughput RNA sequencing data on platform IlluminaHiSeq of hepatocellular carcinoma were downloaded from The Cancer Genome Atlas with 330 primary hepatocellular carcinoma patient samples. Stable key genes with differential expressions were identified with which Kaplan-Meier survival analysis was performed using Cox proportional hazards test in R language. Driver genes influencing the prognosis of this disease were determined using clustering analysis. Functional analysis of driver genes was performed by literature search and Gene Set Enrichment Analysis. Finally, the selected driver genes were verified using external dataset GSE40873. A total of 5781 stable key genes were identified, including 156 genes definitely related to prognoses of hepatocellular carcinoma. Based on the significant key genes, samples were grouped into five clusters which were further integrated into high-and low-risk classes based on clinical features. TMEM88, CCL14, and CLEC3B were selected as driver genes which clustered high-/low-risk patients successfully (generally, p = 0.0005124445). Finally, survival analysis of the high-/low-risk samples from external database illustrated significant difference with p value 0.0198. In conclusion, TMEM88, CCL14, and CLEC3B genes were stable and available in predicting the survival and palindromia time of hepatocellular carcinoma. These genes could function as potential prognostic genes contributing to improve patients' outcomes and survival.
[1]Fourth Peoples Hosp Huaian, Dept Radiol, Huaian, Peoples R China.
[2]Xuzhou Med Univ, Affiliated Huaian Hosp, Dept Med Ultrason, Huaian, Peoples R China.
[3]Second Peoples Hosp Huaian, Huaian, Peoples R China.
[4]Fudan Univ, Peoples Hosp Shanghai 5, Dept Cardiol, Shanghai, Peoples R China.
[5]Hubei Univ Med, Taihe Hosp, Dept Gynecol, Shiyan, Peoples R China.
[6]Hubei Univ Med, Taihe Hosp, Dept Oncol, Shiyan 442000, Hubei, Peoples R China.
[7]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Oral & Craniomaxillofacial, 639 Zhi Zao Ju Rd, Shanghai 200011, Peoples R China.
(8.0+极速模式)