Intrauterine low-protein diet can affect kidney development and hence final nephron number. In this study, we reported that intrauterine low-protein diet can cause congenital anomalies of the kidney and urinary tract (CAKUT) phenotypes, which was dominated by the duplicated collecting system phenotype. At the same time, ectopic ureteric buds were increased under intrauterine low-protein diet and the number of UB branches was reduced in the serum-free culture. Intrauterine low-protein diet can change metanephric gene expression. Slit2/Robo2 and Spry1 expression levels were decreased, Ret expression was increased, and downstream p-Akt activity enhanced with apoptosis abnormal in ureteric bud tissue, which may be the mechanisms that intrauterine low-protein diet causes increased incidence of CAKUT in offspring. Thus, we showed correlation between intrauterine low-protein diet and CAKUT in offspring. (C) 2019 Elsevier Inc. All rights reserved.
[1]Fudan Univ, Shanghai Kidney Dev & Pediat Kidney Dis Res Ctr, Dept Nephrol, Childrens Hosp, Shanghai, Peoples R China;
[2]Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Inst Dev Biol & Mol Med,Collaborat Innovat Ctr Ge, Shanghai, Peoples R China;
[3]Fudan Univ, Natl Ctr Int Res Dev & Dis Inst, Inst Dev Biol & Mol Med, Collaborat Innovat Ctr Gener & Dev,Sch Life Sci, Shanghai, Peoples R China
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