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Genome-Wide Screening Identified That miR-134 Acts as a Metastasis Suppressor by Targeting Integrin beta 1 in Hepatocellular Carcinoma 期刊论文

编号：

415bbce6-2cc0-47c2-95a5-fc88fddca257
作者：

Zha, Ruopeng^[1,2] Guo, Weijie^[1,2] Zhang, Zhenfeng^[2] Qiu, Zhaoping^[2] Wang, Qifeng^[3] Ding, Jie^[2] Huang, Shenglin^[2] Chen, Taoyang^[4] Gu, Jianren^[2] Yao, Ming^[2] He, Xianghuo^[2]
语种：

English
期刊：

PLOS ONE ISSN：1932-6203 2014 年 9 卷 2 期 ; FEB 3
收录：
摘要：

MicroRNAs (miRNAs) are small, single-stranded, non-coding RNAs that play pivotal roles in human cancer development and progression, such as tumor metastasis. Here, we identified the miRNAs that regulate hepatocellular carcinoma (HCC) cell migration by a high-throughput screening method using the classical wound-healing assay with time-lapse video microscopy and validation with a transwell migration assay. Eleven miRNAs (miR-134, -146b-3p, -188-3p, -525-3p, -661, -767-5p, -891a, -891b, -1244, -1247 and miR-1471) were found to promote or inhibit HCC cell migration. Further investigation revealed that miR-134 suppressed the invasion and metastasis of HCC cells in vitro and in vivo, and integrin beta 1 (ITGB1) was a direct and functional target gene of miR-134. Moreover, miR-134 inhibited the phosphorylation of focal adhesion kinase (FAK) and the activation of RhoA downstream of the ITGB1 pathway, thereby decreasing stress fiber formation and cell adhesion in HCC cells. In conclusion, we demonstrated that miR-134 is a novel metastasis suppressor in HCC and could be a potential therapeutic target for the treatment of HCC.
推荐引用方式
GB/T 7714：

Zha Ruopeng,Guo Weijie,Zhang Zhenfeng, et al. Genome-Wide Screening Identified That miR-134 Acts as a Metastasis Suppressor by Targeting Integrin beta 1 in Hepatocellular Carcinoma [J].PLOS ONE,2014,9(2).
APA：

Zha Ruopeng,Guo Weijie,Zhang Zhenfeng,Qiu Zhaoping,&He Xianghuo.(2014).Genome-Wide Screening Identified That miR-134 Acts as a Metastasis Suppressor by Targeting Integrin beta 1 in Hepatocellular Carcinoma .PLOS ONE,9(2).
MLA：

Zha Ruopeng, et al. "Genome-Wide Screening Identified That miR-134 Acts as a Metastasis Suppressor by Targeting Integrin beta 1 in Hepatocellular Carcinoma" .PLOS ONE 9,2(2014).

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