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Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin 期刊论文

编号：

41fa89a7-d340-42aa-aa3a-65d6dc04f92a
作者：

Dong, Caijun^[1] Liu, Xingwang^[2] Wang, Huisheng^[3] Li, Jutao^[4] Dai, Lei^[5] Li, Jun^[6] Xu, Zhen^[1]
语种：

英文
期刊：

ONCOTARGETS AND THERAPY ISSN：1178-6930 2019 年 12 卷 (1947 - 1956)
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关键词：

non-small-cell lung cancer exosomes miR-21 cisplatin resistance PTEN
摘要：

Purpose: To explore the effects of hypoxic non-small-cell lung cancer (NSCLC)-derived exosomes on NSCLC resistance to cisplatin.
Materials and methods: Exosomes were isolated by differential centrifugation and characterized by transmission electron microscope and Western blotting. Quantitative real-time PCR was used to measure miR-21 levels. MTT assays and colony formation assays were performed to investigate the effects of hypoxia-induced exosomes on cisplatin resistance.
Results: Hypoxic NSCLC cell-derived exosomes facilitate normoxic cell resistance to cisplatin. In addition, hypoxia enhanced the miR-21 expression in NSCLC cells and cell-derived exosomes. Interestingly, changes in miR-21 levels in the hypoxia-induced exosomes affected the sensitivity of recipient cells to cisplatin. Mechanically, exosomal miR-21 promoted cisplatin resistance by downregulating phosphatase and tensin homolog (PTEN). The expression of miR-21 in tumor cell lines and clinical NSCLC tumor samples was positively correlated with hypoxiainducible factor-1 alpha and negatively correlated with PTEN. Moreover, high miR-21 expression was associated with shorter median survival period in patients undergoing pharmacotherapy, but no association was observed in patients who were not under pharmacotherapy.
Conclusion: Exosomal miR-21 derived from hypoxic NSCLC cells may promote cisplatin resistance, which indicates that exosomal miR-21 might be a potential biomarker and therapeutic target to address NSCLC chemoresistance.
推荐引用方式
GB/T 7714：

Dong Caijun,Liu Xingwang,Wang Huisheng, et al. Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin [J].ONCOTARGETS AND THERAPY,2019,12:1947-1956.
APA：

Dong Caijun,Liu Xingwang,Wang Huisheng,Li Jutao,&Xu Zhen.(2019).Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin .ONCOTARGETS AND THERAPY,12:1947-1956.
MLA：

Dong Caijun, et al. "Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin" .ONCOTARGETS AND THERAPY 12(2019):1947-1956.
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