Purpose: The purpose of this study is to investigate the effect of differing field strength on the T-2* of cortical bone at 1.5 T and 3 T. Methods: Ultrashort echo time pulse sequences were used to study six bovine and nine human bone samples at 1.5 T and 3 T using single- and bi-component T-2* analysis. Results: On average, the bound water T-2* of bovine bone decreased by 16% (from 0.32 ms at 1.5 T to 0.27 ms at 3 T, P<0.01) and the bound water T-2* of human bone decreased by 21% (from 0.42 ms at 1.5 T to 0.33 ms at 3 T, P<0.01) at the higher field strength. The free water T-2* of bovine bone decreased by 50% (from 4.23 ms at 1.5 T to 2.12 ms at 3 T, P<0.001) and the free water T-2* of human bone decreased by 68% (from 7.65 ms at 1.5 T to 2.46 ms at 3 T, P<0.001) at the higher field strength. Bound and free water fractions showed only minor change with field strength in bovine (< 2%, P>0.05) and human bone (< 4%, P>0.05). Conclusion: Ultrashort echo time bi-component analysis provides consistent bound and free water fractions at 1.5 T and 3 T, thereby allowing field-independent comparisons. Magn Reson Med 71:1075-1081, 2014. (c) 2013 Wiley Periodicals, Inc.