The development of an ultrasensitive nanoprobe is regarded as one of the most promising strategies for the precise diagnosis of tumors. Herein, tanespimycin (17-AAG)-modified polyethylenimine-based gold nanoparticles (AuNPs) co-labeled with a gadolinium (Gd) nanoprobe are constructed for targeted dual-mode computed tomography (CT)/magnetic resonance (MR) imaging. The specific binding of tanespimycin to the N-terminal of heat shock protein 90 (HSP90) endows the modified nanoprobes (NPs) with the ability to up-regulate HSP90 tumor cells and to display excellent X-ray attenuation intensity and T-1 MR imaging performance in an orthotopic hepatocellular carcinoma tumor model. Such an ultrasensitive nanoprobe holds enormous promise for highly efficient tumor diagnosis and dual-mode CT/T-1 positive MR imaging. This study develops a novel strategy to prepare a multifunctional nanoprobe via polyethylenimine nanotechnology. Using this strategy, various dual-mode or multimode nanoprobes for diagnosing different cancers may be designed.
[1]Fudan Univ, Shanghai Inst Med Imaging, Zhongshan Hosp, Dept Intervent, Shanghai 200032, Peoples R China.
[2]Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, 1295 Ding Xi Rd, Shanghai 200050, Peoples R China.
[3]Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China.
(8.0+极速模式)