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Applying massively parallel sequencing to paternity testing on the Ion Torrent Personal Genome Machine 期刊论文

编号：

46379be4-2c72-4ea3-afae-a222eb0d0792
作者：

Li, Hui^[1];Zhao, Xueying^[1];Ma, Ke^[1];Cao, Yu^[2];Zhou, Huaigu^[2];Ping, Yuan^[2];Shao, Chengchen(邵诚臣)^[3]Xie, Jianhui(谢建辉)^[3]Liu, Wenbin^[1];
语种：

English
期刊：

FORENSIC SCIENCE INTERNATIONAL-GENETICS ISSN：1872-4973 2017 年 31 卷 (155 - 159) ; NOV
收录：
关键词：

Forensic genetics Massively parallel sequencing (MPS) Paternity testing Ion Torrent Personal Genome Machine (PGM)
摘要：

Massively parallel sequencing (MPS) is a promising supplementary method for forensic genetics and has gradually been applied to forensic casework. In this study, we applied MPS to forensic casework on an Ion Torrent Personal Genome Machine to evaluate its performance in paternity testing with mismatched STR loci. A total of 15 samples from seven cases containing one mismatched locus by capillary electrophoresis typing were analyzed. Combined paternity index (CPI) and relative chance of paternity were calculated according to the International Society for Forensic Genetics guidelines and the Chinese national standards recommended for paternity testing. With simultaneous analysis of enough STR loci, the results support the certainty of paternity, and the mismatched alleles were considered to be mutations (CPI > 10,000). With the detection of allele sequence structures, the origins of the mutations were inferred in some cases. Meanwhile, nine STRs (CSF1PO, D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D12S391, D21S11 and D4S2408) were found in an increased number of unique alleles and three new alleles in three STRs (D2S441, D21S11, and FGA) that have not been reported before were detected. Therefore, MPS can provide valuable information for forensic genetics research and play a promising role in paternity testing.
推荐引用方式
GB/T 7714：

Li Hui,Zhao Xueying,Ma Ke, et al. Applying massively parallel sequencing to paternity testing on the Ion Torrent Personal Genome Machine [J].FORENSIC SCIENCE INTERNATIONAL-GENETICS,2017,31:155-159.
APA：

Li Hui,Zhao Xueying,Ma Ke,Cao Yu,&Liu Wenbin.(2017).Applying massively parallel sequencing to paternity testing on the Ion Torrent Personal Genome Machine .FORENSIC SCIENCE INTERNATIONAL-GENETICS,31:155-159.
MLA：

Li Hui, et al. "Applying massively parallel sequencing to paternity testing on the Ion Torrent Personal Genome Machine" .FORENSIC SCIENCE INTERNATIONAL-GENETICS 31(2017):155-159.

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