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ALA-PDT suppressing the cell growth and reducing the lipogenesis in human SZ95 sebocytes by mTOR signaling pathway in vitro 期刊论文

编号：

465c3734-6a9b-419b-8138-bf0dced6a285
作者：

Tuo, Jiang^[1];Wang, Qianqian(王倩倩)^[1]Zouboulis, Christos C.^[2,3,4,5];Liu, Ye^[1];Ma, Ying(马英)^[1]Ma, Li(马莉)^[1]Ying, Jiayi^[1];Zhang, Chengfeng(张成锋)^[1]Xiang, Leihong(项蕾红)*^[1]
语种：

English
期刊：

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY ISSN：1572-1000 2017 年 18 卷 (295 - 301) ; JUN
收录：
关键词：

Sebocytes Lipogenesis mTOR 5-Aminolevulinic acid Photodynamic therapy
摘要：

Background: 5-Aminolevulinic acid mediated - photodynamic therapy (ALA-PDT) is known to be effective in treating acne vulgaris and other sebaceous gland-related diseases. However, the therapeutic mechanisms of ALA-PDT still remain undetermined. In this study, we aimed to investigate the effects and mechanisms of ALA-PDT on the cell growth and lipogenesis of human SZ95 sebocytes. Material and methods: Human SZ95 sebocytes were treated with different concentration of ALA-PDT.CCK-8 assay was used to detect cell proliferation activity. Fluorescence microscope and flow cytometry were used to observe the secretion of lipids in SZ95 cells after Nile red staining. Western blotting was used to detect and analyze the protein expression level of P-p70 S6 K/p70 S6 K, P-4E-BP1/4E-BP1, SREBP-1, PPAR gamma, P-mTOR/mTOR, and P-Raptor/Raptor. Mean while, mTOR pathway activator IGF-1 and mTORC1 inhibitor rapamycin were added to observe the interferences on the ALA-PDT treatment of SZ95 cells. Results: ALA-PDT suppressed the cell growth and reduced the secretion of lipids in a dose-dependent manner in SZ95 cells. ALA-PDT reduced the protein levels of P-p70 S6 K (T389), SREBP-1, PPAR-gamma, P-mTOR and P-Raptor. IGF-1 had counter effects on ALA-PDT, and rapamycin enhanced the effects of ALA-PDT in SZ95 cells in suppressing the cell growth and reducing the secretion of lipids. Conclusion: ALA-PDT suppressed the cell growth in SZ95 cells by mTOR-p70 S6K(T389) signaling and reduced the lipogenesis in SZ95 cells by mTOR-SREBP-1/PPAR-gamma signaling. Sebaceous glands atrophy and reduction of sebum secretion after ALA-PDT may be caused by the suppression of lipogenesis and cell growth in sebocytes. (C) 2017 Elsevier B.V. All rights reserved.
推荐引用方式
GB/T 7714：

Tuo Jiang,Wang Qianqian,Zouboulis Christos C., et al. ALA-PDT suppressing the cell growth and reducing the lipogenesis in human SZ95 sebocytes by mTOR signaling pathway in vitro [J].PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY,2017,18:295-301.
APA：

Tuo Jiang,Wang Qianqian,Zouboulis Christos C.,Liu Ye,&Xiang Leihong.(2017).ALA-PDT suppressing the cell growth and reducing the lipogenesis in human SZ95 sebocytes by mTOR signaling pathway in vitro .PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY,18:295-301.
MLA：

Tuo Jiang, et al. "ALA-PDT suppressing the cell growth and reducing the lipogenesis in human SZ95 sebocytes by mTOR signaling pathway in vitro" .PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY 18(2017):295-301.

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