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CDK11(p58) Phosphorylation of PAK1 Ser174 Promotes DLC2 Binding and Roles on Cell Cycle Progression 期刊论文

编号：

46c240f2-988f-417b-a627-873a844ae4cd
作者：

Kong, Xiangfei^[1] Gan, Huachen^[1] Hao, Yuqing^[1] Cheng, Chunming^[1,2] Jiang, Jianhai^[1] Hong, Yi^[1] Yang, Junwu^[1] Zhu, Hao^[1] Chi, Yayun^[1] Yun, Xiaojing^[1] Gu, Jianxin^[1,2]
语种：

English
期刊：

JOURNAL OF BIOCHEMISTRY ISSN：0021-924X 2009 年 146 卷 3 期 (417 - 427) ; SEP
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关键词：

CDK11p58 cell cycle dynein light chain 2 mitosis myosin V PAK1 phosphorylation
摘要：

CDK11(p58), a CDK11 family Ser/Thr kinase, is a G2/M specific protein and contributed to regulation of cell cycle, transcription and apoptotic signal transduction. Recently, CDK11(p58) has been reported to exert important functions in mitotic process, such as the regulation of bipolar spindle formation and sister chromatid cohesion. Here, we identified p21 activated kinase 1 (PAK1) as a new CDK11(p58) substrate and we mapped a new phosphorylation site of Ser174 on PAK1. By mutagenesis, we created PAK1(174A) and PAK1(174E), which mimic the dephosphorylated and phosphorylated form of PAK1; further analysis showed PAK1(174E) could be recruited to myosin V motor complex through binding to dynein light chain 2 (DLC2). PAK1(174E) could accelerate the mitosis progression in a nocodazole blocked cell model, while PAK1(174A) exhibited an opposite role. Our results indicated PAK1 may serve as a downstream effector of CDK11(p58) during mitosis progression.
推荐引用方式
GB/T 7714：

Kong Xiangfei,Gan Huachen,Hao Yuqing, et al. CDK11(p58) Phosphorylation of PAK1 Ser174 Promotes DLC2 Binding and Roles on Cell Cycle Progression [J].JOURNAL OF BIOCHEMISTRY,2009,146(3):417-427.
APA：

Kong Xiangfei,Gan Huachen,Hao Yuqing,Cheng Chunming,&Gu Jianxin.(2009).CDK11(p58) Phosphorylation of PAK1 Ser174 Promotes DLC2 Binding and Roles on Cell Cycle Progression .JOURNAL OF BIOCHEMISTRY,146(3):417-427.
MLA：

Kong Xiangfei, et al. "CDK11(p58) Phosphorylation of PAK1 Ser174 Promotes DLC2 Binding and Roles on Cell Cycle Progression" .JOURNAL OF BIOCHEMISTRY 146,3(2009):417-427.

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