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Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ER alpha 期刊论文

编号：

499b8464-162d-4826-8afe-bc237e546bc0
作者：

Tsang, Wing Pui^[1,2] Zhang, Fengjie^[1,2] He, Qiling^[3] Cai, Waijiao^[4] Huang, Jianhua^[4] Chan, Wai Yee^[1,2] Shen, Ziyin^[4] Wan, Chao^[1,2]
语种：

English
期刊：

SCIENTIFIC REPORTS ISSN：2045-2322 2017 年 7 卷 ; JAN 16
收录：
摘要：

Utilization of small molecules in modulation of stem cell self-renewal is a promising approach to expand stem cells for regenerative therapy. Here, we identify Icaritin, a phytoestrogen molecule enhances self-renewal of mouse embryonic stem cells (mESCs). Icaritin increases mESCs proliferation while maintains their self-renewal capacity in vitro and pluripotency in vivo. This coincides with upregulation of key pluripotency transcription factors OCT4, NANOG, KLF4 and SOX2. The enhancement of mESCs self-renewal is characterized by increased population in S-phase of cell cycle, elevation of Cylin E and Cyclin-dependent kinase 2 (CDK2) and downregulation of p21, p27 and p57. PCR array screening reveals that caudal-related homeobox 2 (Cdx2) and Rbl2/p130 are remarkably suppressed in mESCs treated with Icaritin. siRNA knockdown of Cdx2 or Rbl2/p130 upregulates the expression of Cyclin E, OCT4 and SOX2, and subsequently increases cell proliferation and colony forming efficiency of mESCs. We then demonstrate that Icaritin co-localizes with estrogen receptor alpha (ER alpha) and activates its nuclear translocation in mESCs. The promotive effect of Icaritin on cell cycle and pluripotency regulators are eliminated by siRNA knockdown of ER alpha in mESCs. The results suggest that Icaritin enhances mESCs self-renewal by regulating cell cycle machinery and core pluripotency transcription factors mediated by ER alpha.
推荐引用方式
GB/T 7714：

Tsang Wing Pui,Zhang Fengjie,He Qiling, et al. Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ER alpha [J].SCIENTIFIC REPORTS,2017,7.
APA：

Tsang Wing Pui,Zhang Fengjie,He Qiling,Cai Waijiao,&Wan Chao.(2017).Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ER alpha .SCIENTIFIC REPORTS,7.
MLA：

Tsang Wing Pui, et al. "Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ER alpha" .SCIENTIFIC REPORTS 7(2017).

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