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Brain-selective Kinase 2 (BRSK2) Phosphorylation on PCTAIRE1 Negatively Regulates Glucose-stimulated Insulin Secretion in Pancreatic beta-Cells 期刊论文

编号：

4a8c8ce9-4d98-4958-a865-8e54eb173902
作者：

Chen, XinYa^[1] Gu, XiuTing^[1] Saiyin, Hexige^[1] Wan, Bo^[1] Zhang, YuJing^[2] Li, Jing^[2] Wang, YingLi^[1] Gao, Rui^[1] Wang, YuFan^[3] Dong, WeiPing^[3] Najjar, Sonia M.^[4] Zhang, ChenYu^[2] Ding, HanFei^[5,6] Liu, Jun O.^[7] Yu, Long^[1]
语种：

English
期刊：

JOURNAL OF BIOLOGICAL CHEMISTRY ISSN：0021-9258 2012 年 287 卷 36 期 (30368 - 30375) ; AUG 31
收录：
摘要：

Brain-selective kinase 2 (BRSK2) has been shown to play an essential role in neuronal polarization. In the present study, we show that BRSK2 is also abundantly expressed in pancreatic islets and MIN6 beta-cell line. Yeast two-hybrid screening, GST fusion protein pull-down, and co-immunoprecipitation assays reveal that BRSK2 interacts with CDK-related protein kinase PCTAIRE1, a kinase involved in neurite outgrowth and neurotransmitter release. In MIN6 cells, BRSK2 co-localizes with PCTAIRE1 in the cytoplasm and phosphorylates one of its serine residues, Ser-12. Phosphorylation of PCTAIRE1 by BRSK2 reduces glucose-stimulated insulin secretion (GSIS) in MIN6 cells. Conversely, knockdown of BRSK2 by siRNA increases serum insulin levels in mice. Our results reveal a novel function of BRSK2 in the regulation of GSIS in beta-cells via a PCTAIRE1-dependent mechanism and suggest that BRSK2 is an attractive target for developing novel diabetic drugs.
推荐引用方式
GB/T 7714：

Chen Xin-Ya,Gu Xiu-Ting,Saiyin Hexige, et al. Brain-selective Kinase 2 (BRSK2) Phosphorylation on PCTAIRE1 Negatively Regulates Glucose-stimulated Insulin Secretion in Pancreatic beta-Cells [J].JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(36):30368-30375.
APA：

Chen Xin-Ya,Gu Xiu-Ting,Saiyin Hexige,Wan Bo,&Yu Long.(2012).Brain-selective Kinase 2 (BRSK2) Phosphorylation on PCTAIRE1 Negatively Regulates Glucose-stimulated Insulin Secretion in Pancreatic beta-Cells .JOURNAL OF BIOLOGICAL CHEMISTRY,287(36):30368-30375.
MLA：

Chen Xin-Ya, et al. "Brain-selective Kinase 2 (BRSK2) Phosphorylation on PCTAIRE1 Negatively Regulates Glucose-stimulated Insulin Secretion in Pancreatic beta-Cells" .JOURNAL OF BIOLOGICAL CHEMISTRY 287,36(2012):30368-30375.

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