首页 / 院系成果 / 成果详情页

Retro-Inverso CendR Peptide-Mediated Polyethyleneimine for Intracranial Glioblastoma-Targeting Gene Therapy 期刊论文

编号：

4c563cd1-cb59-49dc-bf8f-8f45e3a8921b
作者：

Wang, Jing(王竞)#^[1]Lei, Yang^[1];Xie, Cao(谢操)^[1]Lu, Weiyue(陆伟跃)^[1]Wagner, Ernst^[1];Xie, Zuoxu^[1];Gao, Jie^[1];Zhang, Xiaoyu^[1];Yan, Zhiqiang^[2];Liu, Min(刘敏)*^[1]
语种：

English
期刊：

BIOCONJUGATE CHEMISTRY ISSN：1043-1802 2014 年 25 卷 2 期 (414 - 423) ; FEB
收录：
摘要：

The development of nonviral gene delivery vectors offers the potential to provide effective treatment for glioblastoma in the form of gene therapy. Here, we report the use of retro-inverso C-end rule (CendR) peptide (D)(RPPREGR) as a targeting ligand to prepare a (D)(RPPREGR)PEG-PEI gene vector. (D)(RPPREGR) peptide specifically recognized the neuropilin-1 receptor that was overexpressed on U87 glioma cells, and showed enhanced tumor spheroid penetration ability. Compared with parental RGERPPR, (D)(RPPREGR) possessed improved biological stability and had a higher affinity for U87 glioma cells; it also showed enhanced penetration of the tumor spheroid. mPEG-PEI/pDNA and (D)(RPPREGR)PEG-PEI/pDNA complexes were prepared and MTT assay results revealed that the cytotoxicity of (D)(RPPREGR)-PEG-PEI complexes was significantly lower than that of PEI complexes, with cell survival rates above 80%. Qualitative and quantitative in vitro transfection results revealed that (D)(RPPREGR)-PEG-PEI complex transfection efficiencies were 1.9 times higher than those of mPEG-PEI. Fluorescent imaging and frozen sections of brain tissue demonstrated that the (D)(RPPREGR) modification improved the in vivo transfection efficiency of mPEG-PEI in nude mice bearing U87 gliomas. An antiglioblastoma assay revealed that (D)(RPPREGR)-PEG-PEI carrying the therapeutic gene pORF-hTRAIL significantly prolonged the survival time of intracranial U87 glioma-bearing mice from 25 to 30 days. Therefore, (D)(RPPREGR)-PEG-PEI appears to be suitable for use as a safe and efficient gene delivery vehicle with potential applications in glioblastoma gene therapy.
推荐引用方式
GB/T 7714：

Wang Jing,Lei Yang,Xie Cao, et al. Retro-Inverso CendR Peptide-Mediated Polyethyleneimine for Intracranial Glioblastoma-Targeting Gene Therapy [J].BIOCONJUGATE CHEMISTRY,2014,25(2):414-423.
APA：

Wang Jing,Lei Yang,Xie Cao,Lu Weiyue,&Liu Min.(2014).Retro-Inverso CendR Peptide-Mediated Polyethyleneimine for Intracranial Glioblastoma-Targeting Gene Therapy .BIOCONJUGATE CHEMISTRY,25(2):414-423.
MLA：

Wang Jing, et al. "Retro-Inverso CendR Peptide-Mediated Polyethyleneimine for Intracranial Glioblastoma-Targeting Gene Therapy" .BIOCONJUGATE CHEMISTRY 25,2(2014):414-423.

浏览次数：9  下载次数：0

分享到：

浏览次数：9

下载次数：0

打印次数：0