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Recovery from acute lung injury can be regulated via modulation of regulatory T cells and Th17 cells 期刊论文

编号：

4fe0b9bd-90ab-4928-86f9-9b44a2a52c1d
作者：

Wang, Linlin(王林林)#^[1]Wang, Xiaocen(王晓岑)^[1]Tong, Lin(童琳)^[1]Wang, Jian(王坚)^[1]Dou, Maosen^[2];Ji, Shimeng^[1];Bi, Jing^[1];Chen, Cuicui(陈翠翠)^[1]Yang, Dong(杨冬)^[1]He, Hong^[3];Bai, Chunxue(白春学)^[1]Zhou, Jian(周建)*^[1]Song, Yuanlin(宋元林)*^[1,4,5]
语种：

English
期刊：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY ISSN：0300-9475 2018 年 88 卷 5 期 ; NOV
收录：
关键词：

acute lung injury disease resolution inflammation regulatory T cells Th17 cells
摘要：

Acute lung injury (ALI) is a severe inflammatory disease, for which no specific treatment exists. The decreased ratio of regulatory T cells (CD4(+)CD25(+)FoxP3 Tregs) and Th17 cells is implicated in ALI and inflammation. We here investigated whether maintaining the balance of CD4(+)CD25(+)Foxp3(+)Tregs and Th17 cells can alleviate lung injury. For CD4(+)CD25(+)FoxP3 Treg depletion, 200g of an anti-CD25 antibody was administered intraperitoneally per mouse on days -3 and -1 before lipopolysaccharide (LPS) instillation. And 150g of TGF- was administered intraperitoneally per mouse on day 0 after LPS instillation. To down-regulate of Th17 cells, 200g per mouse of isotype, IL-17 or IL-22 antibodies were injected intraperitoneally into mice at days 0 after LPS instillation. We detected lung morphology; lung wet-to-dry weight ratio; protein concentration, the count of total cells, neutrophils and macrophages, and cytokines in bronchoalveolar lavage fluid (BALF). And we also evaluated the percentage of CD4(+)CD25(+)Foxp3(+)Tregs in lung, and Th17 cells in lung. CD4(+)CD25(+)Foxp3(+)Tregs depletion via anti-CD25 treatment or TGF- neutralization delayed recovery of ALI. The prolonged inflammation was mainly dominated by neutrophils, macrophages and Th17 cells. Furthermore, inhibition of Th17 cells via monoclonal antibodies against IL-17 and IL-22 alleviated ALI inflammation by inhibiting the recruitment of neutrophils and macrophages, increasing the number of CD4(+)CD25(+)Foxp3(+)Tregs. Our findings support a critical role for CD4(+)CD25(+)Foxp3(+)Tregs in regulating from ALI pathophysiology, and a potential therapeutic role for the inhibition of Th17 cells in ALI treatment. These findings provide a rationale for treating patients with ALI by modulating CD4(+)CD25(+)Foxp3(+)Tregs and Th17 cells.
推荐引用方式
GB/T 7714：

Wang Linlin,Wang Xiaocen,Tong Lin, et al. Recovery from acute lung injury can be regulated via modulation of regulatory T cells and Th17 cells [J].SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2018,88(5).
APA：

Wang Linlin,Wang Xiaocen,Tong Lin,Wang Jian,&Song Yuanlin.(2018).Recovery from acute lung injury can be regulated via modulation of regulatory T cells and Th17 cells .SCANDINAVIAN JOURNAL OF IMMUNOLOGY,88(5).
MLA：

Wang Linlin, et al. "Recovery from acute lung injury can be regulated via modulation of regulatory T cells and Th17 cells" .SCANDINAVIAN JOURNAL OF IMMUNOLOGY 88,5(2018).

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