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Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor 期刊论文

编号：

53504ca8-2e27-4d1f-8a03-e935a25339f6
作者：

Liu, Ying#^[1]Fu, Yajing#^[2,3]Wang, Qian^[4,5];Li, Mushan^[6];Zhou, Zheng^[3];Dabbagh, Deemah^[3];Fu, Chunyan^[1];Zhang, Hang^[1];Li, Shuo^[1];Zhang, Tengjiang^[1];Gong, Jing^[1];Kong, Xiaohui^[1];Zhai, Weiwei^[7,8];Su, Jiaming^[9];Sun, Jianping^[10];Zhang, Yonghong^[10];Yu, XiaoFang^[9];Shao, Zhen^[6];Zhou, Feng*^[4,5]Wu, Yuntao*^[3]Tan, Xu*^[1]
语种：

英文
期刊：

NATURE MICROBIOLOGY ISSN：2058-5276 2019 年 4 卷 5 期 (813 - 825) ; MAY
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摘要：

Human immunodeficiency virus (HIV) actively modulates the protein stability of host cells to optimize viral replication. To systematically examine this modulation in HIV infection, we used isobaric tag-based mass spectrometry to quantify changes in the abundance of over 14,000 proteins during HIV-1 infection of human primary CD4(+) T cells. We identified P-selectin glycoprotein ligand 1 (PSGL-1) as an HIV-1 restriction factor downregulated by HIV-1 Vpu, which binds to PSGL-1 and induces its ubiquitination and degradation through the ubiquitin ligase SCF beta-TrCP2. PSGL-1 is induced by interferon-gamma in activated CD4(+) T cells to inhibit HIV-1 reverse transcription and potently block viral infectivity by incorporating in progeny virions. This infectivity block is antagonized by Vpu via PSGL-1 degradation. We further show that PSGL-1 knockdown can significantly abolish the anti-HIV activity of interferon-gamma in primary CD4(+) T cells. Our study identifies an HIV restriction factor and a key mediator of interferon-gamma's anti-HIV activity.
推荐引用方式
GB/T 7714：

Liu Ying,Fu Yajing,Wang Qian, et al. Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor [J].NATURE MICROBIOLOGY,2019,4(5):813-825.
APA：

Liu Ying,Fu Yajing,Wang Qian,Li Mushan,&Tan Xu.(2019).Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor .NATURE MICROBIOLOGY,4(5):813-825.
MLA：

Liu Ying, et al. "Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor" .NATURE MICROBIOLOGY 4,5(2019):813-825.
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