首页 / 院系成果 / 成果详情页

Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein 期刊论文

编号：

541d2e0e-eeb8-4573-b6b7-7cda80fa05ec
作者：

Huang, Jing^[1] Wu, Tong^[1] Guo, Zheng^[1] Lou, Tiantian^[1] Yu, Shaoning^[1] Gong, Weimin^[1] Ji, Chaoneng^[1]
语种：

English
期刊：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS ISSN：2053-230X 2013 年 69 卷 (1436 - 1439) ; DEC
收录：
摘要：

The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. Wild-type CRP can bind to special DNA sequences in the presence of cAMP. The substitution of Asp53 by His results in the CRP* phenotype, which does not require exogenous cAMP. In the present study, the D53H CRP mutant was overexpressed, purified and crystallized. cAMP-free D53H CRP crystals were obtained and diffracted to a resolution of 2.9 angstrom. Based on the systematic absences of the crystals, the space group is likely to be P2(1)2(1)2(1), with unit-cell parameters a = 76.66, b = 152.14, c = 176.11 angstrom. The asymmetric unit was confirmed to contain four protein dimers, with a Matthews coefficient of 2.71 angstrom(3) Da(-1) and a solvent content of 54.68%.
推荐引用方式
GB/T 7714：

Huang Jing,Wu Tong,Guo Zheng, et al. Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein [J].ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2013,69:1436-1439.
APA：

Huang Jing,Wu Tong,Guo Zheng,Lou Tiantian,&Ji Chaoneng.(2013).Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein .ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,69:1436-1439.
MLA：

Huang Jing, et al. "Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein" .ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 69(2013):1436-1439.

浏览次数：1  下载次数：0

分享到：

浏览次数：1

下载次数：0

打印次数：0