Proanthocyanidin A2 (PA2), one of A-type proanthocyanidins, has been shown to harbor a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, anti-HIV, anti-CDV and anti-alpha-glucosidase activities. However, little is known about the role for PA2 in regulating PDGF-induced VSMC proliferation and migration. In the present study, we investigated the possible effects of PA2 on PDGF-BB-induced proliferation, migration and inflammation in VSMCs in vitro to mimic a postangioplasty PDGF shedding condition. Herein, the data clearly show that PA2 markedly inhibited proliferation, migration and inflammatory responses at 0-30 mu g/ml concentration in VSMCs in vitro. 10-30 mu g/ml PA2 inhibited PDGF-mediated NAD(P)H oxidase activation and intracellular ROS formation in VSMCs. Furthermore, the effects exerted by PA2 involve the participation of KDR and Jak-2/STAT-3/cPLA(2) signaling pathways. These data also highlight the possible therapeutic use of PA2 in vascular proliferative diseases, where abnormal proliferation and migration play important pathological roles.
[1]Huashan Hosp North, Dept Pharm, Shanghai 201907, Peoples R China;
[2]Huashan Hosp North, Dept Gen Surg, Shanghai 201907, Peoples R China;
[3]Huashan Hosp, Dept Cardiothorac Surg, Shanghai 200040, Peoples R China;
[4]Brunswick Labs China, Suzhou Ind Pk, Suzhou 215021, Peoples R China;
[5]Xuhui Dist Cent Hosp, Pharm Dept, 966 Huai Hai M Rd, Shanghai 200031, Peoples R China;
[6]Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China
(8.0+极速模式)