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Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus 期刊论文

编号：

5a969656-87f8-4e72-8ba7-845cdc5392ad
作者：

Zhang, Naru^[1];Channappanavar, Rudragouda^[2];Ma, Cuiqing^[1];Wang, Lili^[1];Tang, Jian^[1,3,8];Garron, Tania^[4,5];Tao, Xinrong^[4,5];Tasneem, Sumaiya^[1];Lu, Lu(陆路)^[6,7]Tseng, ChienTe K.^[4,5];Zhou, Yusen^[3,8];Perlman, Stanley^[2];Jiang, Shibo(姜世勃)^[1,6,7]Du, Lanying^[1];
语种：

English
期刊：

CELLULAR & MOLECULAR IMMUNOLOGY ISSN：1672-7681 2016 年 13 卷 2 期 (180 - 190) ; MAR
收录：
关键词：

adjuvant effects MERS MERS-CoV receptor-binding domain subunit vaccine
摘要：

Middle East respiratory syndrome (MERS), an emerging infectious disease caused by MERS coronavirus (MERS-CoV), has garnered worldwide attention as a consequence of its continuous spread and pandemic potential, making the development of effective vaccines a high priority. We previously demonstrated that residues 377-588 of MERS-CoV spike (S) protein receptor-binding domain (RBD) is a very promising MERS subunit vaccine candidate, capable of inducing potent neutralization antibody responses. In this study, we sought to identify an adjuvant that optimally enhanced the immunogenicity of S377-588 protein fused with Fc of human IgG (S377-588-Fc). Specifically, we compared several commercially available adjuvants, including Freund's adjuvant, aluminum, Monophosphoryl lipid A, Montanide ISA51 and MF59 with regard to their capacity to enhance the immunogenicity of this subunit vaccine. In the absence of adjuvant, S377-588-Fc alone induced readily detectable neutralizing antibody and T-cell responses in immunized mice. However, incorporating an adjuvant improved its immunogenicity. Particularly, among the aforementioned adjuvants evaluated, MF59 is the most potent as judged by its superior ability to induce the highest titers of IgG, IgG1 and IgG2a subtypes, and neutralizing antibodies. The addition of MF59 significantly augmented the immunogenicity of S377-588-Fc to induce strong IgG and neutralizing antibody responses as well as protection against MERS-CoV infection in mice, suggesting that MF59 is an optimal adjuvant for MERS-CoV RBD-based subunit vaccines.
推荐引用方式
GB/T 7714：

Zhang Naru,Channappanavar Rudragouda,Ma Cuiqing, et al. Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus [J].CELLULAR & MOLECULAR IMMUNOLOGY,2016,13(2):180-190.
APA：

Zhang Naru,Channappanavar Rudragouda,Ma Cuiqing,Wang Lili,&Du Lanying.(2016).Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus .CELLULAR & MOLECULAR IMMUNOLOGY,13(2):180-190.
MLA：

Zhang Naru, et al. "Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus" .CELLULAR & MOLECULAR IMMUNOLOGY 13,2(2016):180-190.

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